{"doi":"10.3791/62001","title":"CRISPR/Cas9 Editing of the <em>C. elegans rbm-3.2</em> Gene using the <em>dpy-10</em> Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.","abstract":null,"journal":"Journal of Visualized Experiments","year":2020,"id":604839,"datarank":0.38476558499707186,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.07284935374509643,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.07284935374509643,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":5,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":829379,"name":"Mary Bergwell","orcid":"0000-0003-2139-6756","position":1,"is_corresponding":false},{"id":830660,"name":"Ellie Smith","orcid":null,"position":2,"is_corresponding":false},{"id":830662,"name":"Danita Mathew","orcid":null,"position":3,"is_corresponding":false},{"id":829377,"name":"Jyoti Iyer","orcid":"0000-0002-0942-6919","position":4,"is_corresponding":false},{"id":744608,"name":"Amy Smith","orcid":"0000-0003-4430-7314","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CRISPR/Cas9 Editing of the <em>C. elegans rbm-3.2</em> Gene using the <em>dpy-10</em> Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.","abstract":"The bacterial Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Streptococcus pyogenes CRISPR-associated protein (Cas) system has been harnessed by researchers to study important biologically relevant problems. The unparalleled power of the CRISPR/Cas genome editing method allows researchers to precisely edit any locus of their choosing, thereby facilitating an increased understanding of gene function. Several methods for editing the C. elegans genome by CRISPR/Cas9 have been described previously. Here, we discuss and demonstrate a method which utilizes in vitro assembled ribonucleoprotein complexes and the dpy-10 co-CRISPR marker for screening. Specifically, in this article, we go through the step-by-step process of introducing premature stop codons into the C. elegans rbm-3.2 gene by homology-directed repair using this method of CRISPR/Cas9 editing. This relatively simple editing method can be used to study the function of any gene of interest and allows for the generation of homozygous-edited C. elegans by CRISPR/Cas9 editing in less than two weeks.","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33369608","pmcid":null,"openalex_id":"https://openalex.org/W3111242925","authors":[],"funders":[],"total_grants":0,"fwci":0.2237,"citation_percentile":0.5032075,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.jove.com/pdf/62001/crisprcas9-editing-c-elegans-rbm-32-gene-using-dpy-10-co-crispr","host_type":"journal"},{"url":"https://www.jove.com/pdf/62001/crisprcas9-editing-c-elegans-rbm-32-gene-using-dpy-10-co-crispr","host_type":"publisher"},{"url":"https://www.jove.com/t/62001/crisprcas9-editing-c-elegans-rbm-32-gene-using-dpy-10-co-crispr","host_type":"publisher"},{"url":"https://doi.org/10.3791/62001","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33369608","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","Genetics, Aging, and Longevity in Model Organisms","Innovation and Socioeconomic Development","Animals","Base Sequence","CRISPR-Associated Protein 9","CRISPR-Cas Systems","Caenorhabditis elegans","Caenorhabditis elegans Proteins","Collagen","DNA Primers","Genetic Testing","Gonads","Homozygote","Microinjections","RNA Editing","RNA, Ribosomal","Restriction Mapping","Ribonucleoproteins","Streptococcus pyogenes"],"mesh_terms":["CRISPR-Associated Protein 9","Animals","Base Sequence","Collagen","Genetic Testing","Gonads","Homozygote","Microinjections","Ribonucleoproteins","RNA, Ribosomal","Streptococcus pyogenes","Restriction Mapping","Caenorhabditis elegans","RNA Editing","DNA Primers","Caenorhabditis elegans Proteins","CRISPR-Cas Systems"],"keywords":["CRISPR","Genome editing","Cas9","Biology","CRISPR interference","Genetics","Gene","Trans-activating crRNA","Guide RNA","Computational biology","Caenorhabditis elegans"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T01:02:22.515383Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}