{"doi":"10.3791/52785","title":"Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration","abstract":null,"journal":"Journal of Visualized Experiments","year":2015,"id":607853,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":297454,"name":"Mehwish Khaliq","orcid":"0000-0001-8757-8013","position":1,"is_corresponding":false},{"id":277666,"name":"Sungjin Ko","orcid":"0000-0002-6079-5939","position":2,"is_corresponding":false},{"id":275468,"name":"Juhoon So","orcid":"0000-0003-3469-6686","position":3,"is_corresponding":false},{"id":275471,"name":"Donghun Shin","orcid":"0000-0002-7975-9014","position":4,"is_corresponding":false},{"id":1560897,"name":"Tae-Young Choi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration","abstract":"The liver has a great capacity to regenerate. Hepatocytes, the parenchymal cells of the liver, can regenerate in one of two ways: hepatocyte- or biliary-driven liver regeneration. In hepatocyte-driven liver regeneration, regenerating hepatocytes are derived from preexisting hepatocytes, whereas, in biliary-driven regeneration, regenerating hepatocytes are derived from biliary epithelial cells (BECs). For hepatocyte-driven liver regeneration, there are excellent rodent models that have significantly contributed to the current understanding of liver regeneration. However, no such rodent model exists for biliary-driven liver regeneration. We recently reported on a zebrafish liver injury model in which BECs extensively give rise to hepatocytes upon severe hepatocyte loss. In this model, hepatocytes are specifically ablated by a pharmacogenetic means. Here we present in detail the methods to ablate hepatocytes and to analyze the BEC-driven liver regeneration process. This hepatocyte-specific ablation model can be further used to discover the underlying molecular and cellular mechanisms of biliary-driven liver regeneration. Moreover, these methods can be applied to chemical screens to identify small molecules that augment or suppress liver regeneration.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26065829","pmcid":"PMC4542854","openalex_id":"https://openalex.org/W344705032","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK101426","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK101426","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"F31 DK105714","title":null},{"funder_name":"NIBIB NIH HHS","grant_id":"T32 EB001026","title":null}],"total_grants":4,"fwci":1.0305,"citation_percentile":0.74328199,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2017,"count":1},{"year":2018,"count":5},{"year":2019,"count":2},{"year":2020,"count":2},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://www.jove.com/video/52785/hepatocyte-specific-ablation-zebrafish-to-study-biliary-driven-liver","host_type":"publisher"},{"url":"https://doi.org/10.3791/52785","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26065829","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4542854","host_type":"repository"}],"fields_of_study":["Liver physiology and pathology","Pancreatic function and diabetes","Organ Transplantation Techniques and Outcomes","Ablation Techniques","Animals","Animals, Genetically Modified","Biliary Tract","Female","Hepatocytes","Liver","Liver Regeneration","Male","Metronidazole","Models, Animal","Nitroreductases","Zebrafish"],"mesh_terms":["Animals","Biliary Tract","Female","Liver","Liver Regeneration","Male","Metronidazole","Nitroreductases","Zebrafish","Hepatocytes","Models, Animal","Animals, Genetically Modified","Ablation Techniques"],"keywords":["Hepatocyte","Regeneration (biology)","Liver regeneration","Zebrafish","Biology","Cell biology","Liver cytology","Endocrinology","Liver metabolism","Biochemistry","In vitro","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T07:06:28.307344Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}