{"doi":"10.3791/52619","title":"Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques","abstract":null,"journal":"Journal of Visualized Experiments","year":2015,"id":604807,"datarank":9.434095271304397,"base_score":5.170483995038151,"endowment":5.170483995038151,"self_citation_contribution":0.7755725992557229,"citation_network_contribution":8.658522672048674,"self_endowment_contribution":0.7755725992557229,"citer_contribution":8.658522672048674,"corpus_percentile":null,"corpus_rank":null,"citation_count":175,"citer_count":175,"citers_with_citation_signal":148,"citers_with_endowment":148,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1551880,"name":"Oumaima Stambouli","orcid":null,"position":1,"is_corresponding":false},{"id":1551881,"name":"R. Stefan Ross","orcid":null,"position":2,"is_corresponding":false},{"id":1551879,"name":"Nico Grüner","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques","abstract":"The idea of collecting blood on a paper card and subsequently using the dried blood spots (DBS) for diagnostic purposes originated a century ago. Since then, DBS testing for decades has remained predominantly focused on the diagnosis of infectious diseases especially in resource-limited settings or the systematic screening of newborns for inherited metabolic disorders and only recently have a variety of new and innovative DBS applications begun to emerge. For many years, pre-analytical variables were only inappropriately considered in the field of DBS testing and even today, with the exception of newborn screening, the entire pre-analytical phase, which comprises the preparation and processing of DBS for their final analysis has not been standardized. Given this background, a comprehensive step-by-step protocol, which covers al the essential phases, is proposed, i.e., collection of blood; preparation of blood spots; drying of blood spots; storage and transportation of DBS; elution of DBS, and finally analyses of DBS eluates. The effectiveness of this protocol was first evaluated with 1,762 coupled serum/DBS pairs for detecting markers of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus infections on an automated analytical platform. In a second step, the protocol was utilized during a pilot study, which was conducted on active drug users in the German cities of Berlin and Essen.","is_dataset_classified":null,"base_score":5.170483995038151,"endowment":5.170483995038151,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25867233","pmcid":"PMC4397000","openalex_id":"https://openalex.org/W2031465040","authors":[],"funders":[],"total_grants":0,"fwci":3.9174,"citation_percentile":0.94323733,"influential_citations":0,"citation_trend":[{"year":2016,"count":8},{"year":2017,"count":9},{"year":2018,"count":14},{"year":2019,"count":17},{"year":2020,"count":22},{"year":2021,"count":27},{"year":2022,"count":23},{"year":2023,"count":18},{"year":2024,"count":22},{"year":2025,"count":14},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://www.jove.com/pdf/52619/dried-blood-spots-preparing-processing-for-use-immunoassays-molecular","host_type":"journal"},{"url":"https://www.jove.com/pdf/52619/dried-blood-spots-preparing-processing-for-use-immunoassays-molecular","host_type":"publisher"},{"url":"http://www.jove.com/video/52619/dried-blood-spots-preparing-processing-for-use-immunoassays-molecular","host_type":"publisher"},{"url":"https://doi.org/10.3791/52619","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25867233","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4397000","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4397000","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4397000?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Biosimilars and Bioanalytical Methods","Blood groups and transfusion","Cytomegalovirus and herpesvirus research"],"mesh_terms":["Hepatitis B virus","Hepatitis C","Humans","Immunoassay","Pilot Projects","Virus Diseases","HIV Infections","Hepacivirus","Hepatitis B, Chronic","Dried Blood Spot Testing"],"keywords":["Dried blood","Dried blood spot","Human immunodeficiency virus (HIV)","Spots","Protocol (science)","Medicine","Blood collection","Computer science","Virology","Pathology","Medical emergency","Chemistry","Chromatography"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T00:57:21.980536Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}