{"doi":"10.3791/50617","title":"Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research","abstract":null,"journal":"Journal of Visualized Experiments","year":2013,"id":640519,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":970426,"name":"Britton J. Grasperge","orcid":"0000-0001-5592-9413","position":1,"is_corresponding":false},{"id":970865,"name":"Mary B. Jacobs","orcid":null,"position":2,"is_corresponding":false},{"id":1107845,"name":"Mario T. Philipp","orcid":null,"position":3,"is_corresponding":false},{"id":536215,"name":"Monica E. Embers","orcid":"0000-0003-4051-7592","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research","abstract":"Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the attachment and blood feeding of Ixodes species ticks on mammalian hosts. In nature, this zoonotic bacterial pathogen may use a variety of reservoir hosts, but the white-footed mouse (Peromyscus leucopus) is the primary reservoir for larval and nymphal ticks in North America. Humans are incidental hosts most frequently infected with B. burgdorferi by the bite of ticks in the nymphal stage. B. burgdorferi adapts to its hosts throughout the enzootic cycle, so the ability to explore the functions of these spirochetes and their effects on mammalian hosts requires the use of tick feeding. In addition, the technique of xenodiagnosis (using the natural vector for detection and recovery of an infectious agent) has been useful in studies of cryptic infection. In order to obtain nymphal ticks that harbor B. burgdorferi, ticks are fed live spirochetes in culture through capillary tubes. Two animal models, mice and nonhuman primates, are most commonly used for Lyme disease studies involving tick feeding. We demonstrate the methods by which these ticks can be fed upon, and recovered from animals for either infection or xenodiagnosis.","is_dataset_classified":null,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24022694","pmcid":"PMC3857350","openalex_id":"https://openalex.org/W2077478844","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"8 P20 GM103458-09","title":null},{"funder_name":"NIH HHS","grant_id":"P51OD011104/P51RR000164","title":null},{"funder_name":"NIH HHS","grant_id":"P51 OD011104","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"P51 RR000164","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"P20 GM103458","title":null},{"funder_name":"NIH HHS","grant_id":"T32 OD011064","title":null}],"total_grants":6,"fwci":0.8868,"citation_percentile":0.70264976,"influential_citations":0,"citation_trend":[{"year":2015,"count":2},{"year":2016,"count":2},{"year":2017,"count":2},{"year":2019,"count":3},{"year":2020,"count":4},{"year":2021,"count":3},{"year":2022,"count":4},{"year":2024,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://www.jove.com/video/50617/feeding-ticks-on-animals-for-transmission-xenodiagnosis-lyme-disease","host_type":"publisher"},{"url":"https://doi.org/10.3791/50617","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24022694","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3857350","host_type":"repository"}],"fields_of_study":["Vector-borne infectious diseases","Viral Infections and Vectors","Vector-Borne Animal Diseases","Animals","Borrelia burgdorferi","Disease Models, Animal","Ixodidae","Lyme Disease","Macaca mulatta","Mice","Xenodiagnosis"],"mesh_terms":["Animals","Disease Models, Animal","Lyme Disease","Macaca mulatta","Xenodiagnosis","Borrelia burgdorferi","Ixodidae","Mice"],"keywords":["Xenodiagnosis","Enzootic","Borrelia burgdorferi","Biology","Lyme disease","Ixodes scapularis","Ixodes","Tick","Peromyscus","Virology","Vector (molecular biology)","Transmission (telecommunications)","Zoology","Microbiology","Ixodidae","Immunology","Parasite hosting","Virus","Trypanosoma cruzi"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T12:28:19.735724Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}