{"doi":"10.3748/wjg.v12.i33.5306","title":"<i>H pylori</i>receptor MHC class II contributes to the dynamic gastric epithelial apoptotic response","abstract":null,"journal":"World Journal of Gastroenterology","year":2006,"id":595401,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1524651,"name":"David A Bland","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"<i>H pylori</i>receptor MHC class II contributes to the dynamic gastric epithelial apoptotic response","abstract":"To investigate the role of MHC class II in the modulation of gastric epithelial cell apoptosis induced by H pylori infection.After stimulating a human gastric epithelial cell line with bacteria or agonist antibodies specific for MHC class II and CD95, the quantitation of apoptotic and anti-apoptotic events, including caspase activation, BCL-2 activation, and FADD recruitment, was performed with a fluorometric assay, a cytometric bead array, and confocal microscopy, respectively.Pretreatment of N87 cells with the anti-MHC class II IgM antibody RFD1 resulted in a reduction in global caspase activation at 24 h of H pylori infection. When caspase 3 activation was specifically measured, crosslinking of MHC class II resulted in markedly reduced caspase activation, while simple ligation of MHC class II did not. Crosslinking of MHC class II also resulted in an increased activation of the anti-apoptosis molecule BCL-2 compared to simple ligation. Confocal microscope analysis demonstrated that the pretreatment of gastric epithelial cells with a crosslinking anti-MHC class II IgM blocked the recruitment of FADD to the cell surface.The ability of MHC class II to modulate gastric epithelial apoptosis is at least partially dependent on its crosslinking. The crosslinking of this molecule has anti-apoptotic effects during the earlier time points of H pylori infection. This effect is possibly mediated by the ability of MHC class II to modulate the activation of the pro-apoptotic receptor Fas by blocking the recruitment of the accessory molecule FADD, and this delay in apoptosis induction could allow for prolonged cytokine secretion by H pylori-infected gastric epithelial cells.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16981259","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"5T32AI007536-09","title":"TRAINING IN EMERGING AND RE-EMERGING INFECTIOUS DISEASES"},{"funder_name":"National Institutes of Health","grant_id":"1R01DK050669-01","title":"IMPAIRED TUMOR IMMUNITY DURING H PYLORI INFECTION"},{"funder_name":"NIAID NIH HHS","grant_id":"T32 AI00736-06","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"T32 AI007536","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK050669","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"P30 DK056338","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK56338","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK50669","title":null}],"total_grants":8,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by-nc","oa_locations":[{"url":"https://www.wjgnet.com/1007-9327/full/v12/i33/5306.htm","host_type":"publisher"},{"url":"https://doi.org/10.3748/wjg.v12.i29.4689","host_type":""},{"url":"https://doi.org/10.3748/wjg.v12.i33.5306","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/16937440","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/16981259","host_type":""},{"url":"https://dx.doi.org/10.3748/wjg.v12.i33.5306","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences","0303 health sciences","Apoptosis","Caspase 3","Caspases","Cell Line","Cell Line, Tumor","DNA Fragmentation","Enzyme Activation","Epithelium","Gastric Mucosa","Genes, MHC Class II","Helicobacter Infections","Helicobacter pylori","Histocompatibility Antigens Class II","Humans","Microscopy, Confocal","Proto-Oncogene Proteins c-bcl-2","fas Receptor"],"mesh_terms":["Apoptosis","Caspase 3","Caspases","Cell Line","Cell Line, Tumor","DNA Fragmentation","Enzyme Activation","Epithelium","Gastric Mucosa","Genes, MHC Class II","Helicobacter Infections","Helicobacter pylori","Histocompatibility Antigens Class II","Humans","Microscopy, Confocal","Proto-Oncogene Proteins c-bcl-2","fas Receptor"],"keywords":["Fas-Associated Death Domain Protein","Genes, MHC Class II","Apoptosis","DNA Fragmentation","Epithelium","Antibodies","Cell Line","Helicobacter Infections","Cell Line, Tumor","Humans","fas Receptor","Adaptor Proteins, Signal Transducing","Microscopy, Confocal","Helicobacter pylori","Caspase 3","Stomach","Histocompatibility Antigens Class II","Epithelial Cells","Enzyme Activation","Proto-Oncogene Proteins c-bcl-2","Gastric Mucosa","Caspases"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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