{"doi":"10.3725/jjn.40.13","title":"A DNA extraction method with SDS from single nematodes for direct application to PCR amplification","abstract":null,"journal":"Nematological Research","year":2010,"id":590867,"datarank":0.5810893926527144,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.20835339518451426,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.20835339518451426,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":7,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1511702,"name":"Hiromichi Sakai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A DNA extraction method with SDS from single nematodes for direct application to PCR amplification","abstract":"Various methods for extracting DNA from single nematodes for PCR amplification have been developed by many authors. Such techniques generally do not include a purification step, and direct application of crude lysate to PCR amplification is usual. SDS is not used in DNA extraction methods for PCR amplification from single nematodes, although it is a common detergent used in DNA extraction. This is because SDS has an inhibitory effect on PCR (Gelfand, 1989). The author developed an SDS-based DNA extraction method from single nematodes, where the crude lysate can be applied directly to PCR amplification, and reports it here.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26657633","pmcid":null,"openalex_id":"https://openalex.org/W2325665104","authors":[],"funders":[],"total_grants":0,"fwci":1.1292,"citation_percentile":0.81228205,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"gold","license":null,"oa_locations":[{"url":"https://www.jstage.jst.go.jp/article/jjn/40/1/40_1_13/_pdf","host_type":"journal"},{"url":"https://www.jstage.jst.go.jp/article/jjn/40/1/40_1_13/_pdf","host_type":"publisher"},{"url":"https://doi.org/10.3725/jjn.40.13","host_type":"journal"}],"fields_of_study":["Insect symbiosis and bacterial influences","Environmental DNA in Biodiversity Studies","Nematode management and characterization studies"],"mesh_terms":[],"keywords":["DNA extraction","Lysis","DNA","Multiple displacement amplification","Polymerase chain reaction","Applications of PCR","Extraction (chemistry)","Biology","Chromatography","Primer dimer","Molecular biology","Lysis buffer","Alkaline lysis","Chemistry","Digital polymerase chain reaction","Gene","Genetics","Multiplex polymerase chain reaction","Plasmid"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-25T12:52:10.031688Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}