{"doi":"10.3410/f.737122326.793579896","title":"Faculty Opinions recommendation of An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms.","abstract":"Here we report a rapid CRISPR/Cas9-mediated gene knock-in strategy that utilizes Cas9 ribonucleoprotein and 5' modified dsDNA donors with 50 bp homology arms and achieved unprecedented 65%/40% KI rates for 0.7 Kb/2.5 Kb inserts, respectively, in HEK293T cells. The identified 5' end modification led to up to 5-fold increase in gene KI rates at various genomic loci in human cancer and stem cells.","journal":"Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature","year":2020,"id":133188,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9492,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":347303,"name":"Yousin Suh","orcid":"0000-0002-6014-3084","position":0,"is_corresponding":true}],"reference_count":1,"raw_metadata":null,"created_at":"2026-07-18T23:16:17.577688Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}