{"doi":"10.3410/f.736650692.793575792","title":"Faculty Opinions recommendation of DART-seq: an antibody-free method for global m6A detection.","abstract":"m 6 A is a widespread RNA modification which influences nearly every aspect of the mRNA life cycle. Our understanding of m 6 A has been facilitated by the development of global m 6 A mapping methods, which use antibodies to immunoprecipitate methylated RNA. However, these methods have several limitations, including high input RNA requirements and cross-reactivity to other RNA modifications. Here, we present DART-Seq (deamination adjacent to RNA modification targets), an antibody-free method for detecting m 6 A sites. In DART-Seq, the cytidine deaminase APOBEC1 is fused to the m 6 A-binding YTH domain. APOBEC1-YTH expression in cells induces C to U deamination at sites adjacent to m 6 A residues, which are detected using standard RNA-Seq. DART-Seq identifies thousands of m 6 A sites in cells from as little as 10 nanograms of total RNA and can detect m 6 A accumulation in cells over time. Additionally, we use long-read DART-Seq to gain new insights into m 6 A distribution along the length of individual transcripts.","journal":"Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature","year":2020,"id":134351,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9622,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":267557,"name":"Lloyd M. Smith","orcid":"0000-0002-6652-8639","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:16:24.540681Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}