{"doi":"10.3410/f.727483654.793582001","title":"Faculty Opinions recommendation of An approach to spatiotemporally resolve protein interaction networks in living cells.","abstract":"Cells operate through protein interaction networks organized in space and time. Here, we describe an approach to resolve both dimensions simultaneously by using proximity labeling mediated by engineered ascorbic acid peroxidase (APEX). APEX has been used to capture entire organelle proteomes with high temporal resolution, but its breadth of labeling is generally thought to preclude the higher spatial resolution necessary to interrogate specific protein networks. We provide a solution to this problem by combining quantitative proteomics with a system of spatial references. As proof of principle, we apply this approach to interrogate proteins engaged by G-protein-coupled receptors as they dynamically signal and traffic in response to ligand-induced activation. The method resolves known binding partners, as well as previously unidentified network components. Validating its utility as a discovery pipeline, we establish that two of these proteins promote ubiquitin-linked receptor downregulation after prolonged activation.Copyright © 2017 Elsevier Inc. All rights reserved. PMID: 28388416 Funding information This work was supported by: NIDA NIH HHS, United States Grant ID: R01 DA010711 NIDA NIH HHS, United States Grant ID: R01 DA012864 NIAID NIH HHS, United States Grant ID: P01 AI063302 NIAID NIH HHS, United States Grant ID: U19 AI106754 NIAID NIH HHS, United States Grant ID: P30 AI027763 NIAID NIH HHS, United States Grant ID: U19 AI118610 NIGMS NIH HHS, United States Grant ID: P50 GM082250 NCI NIH HHS, United States Grant ID: R01 CA186568 NIDA NIH HHS, United States Grant ID: R29 DA010711 NIDA NIH HHS, United States Grant ID: F32 DA038947 NIAID NIH HHS, United States Grant ID: R01 AI120694 NIDA NIH HHS, United States Grant ID: R37 DA010711 NIGMS NIH HHS, United States Grant ID: T32 GM007810 NHLBI NIH HHS, United States Grant ID: P01 HL089707 NIDA NIH HHS, United States Grant ID: P01 DA010154 More Less keyboard_arrow_down","journal":"Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature","year":2021,"id":228601,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9451,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":319976,"name":"Emmanuel Darcq","orcid":"0000-0002-8377-8015","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:54:54.016932Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}