{"doi":"10.3390/vaccines10111935","title":"Single-Shot ChAd3-MARV Vaccine in Modified Formulation Buffer Shows 100% Protection of NHPs","abstract":"Marburg virus (MARV) is a virus of high human consequence with a case fatality rate of 24–88%. The global health and national security risks posed by Marburg virus disease (MVD) underscore the compelling need for a prophylactic vaccine, but no candidate has yet reached regulatory approval. Here, we evaluate a replication-defective chimpanzee adenovirus type 3 (ChAd3)-vectored MARV Angola glycoprotein (GP)-expressing vaccine against lethal MARV challenge in macaques. The ChAd3 platform has previously been reported to protect against the MARV-related viruses, Ebola virus (EBOV) and Sudan virus (SUDV), and MARV itself in macaques, with immunogenicity demonstrated in macaques and humans. In this study, we present data showing 100% protection against MARV Angola challenge (versus 0% control survival) and associated production of GP-specific IgGs generated by the ChAd3-MARV vaccine following a single dose of 1 × 1011 virus particles prepared in a new clinical formulation buffer designed to enhance product stability. These results are consistent with previously described data using the same vaccine in a different formulation and laboratory, demonstrating the reproducible and robust protective efficacy elicited by this promising vaccine for the prevention of MVD. Additionally, a qualified anti-GP MARV IgG ELISA was developed as a critical pre-requisite for clinical advancement and regulatory approval.","journal":"Vaccines","year":2022,"id":262617,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9548,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":918994,"name":"Thomas H. King","orcid":null,"position":1,"is_corresponding":false},{"id":229221,"name":"Kendra J. Alfson","orcid":null,"position":2,"is_corresponding":false},{"id":918442,"name":"Katie A. Albanese","orcid":"0000-0001-6282-541X","position":3,"is_corresponding":false},{"id":488777,"name":"Julianne Smith","orcid":"0000-0002-2536-5933","position":4,"is_corresponding":false},{"id":863781,"name":"Paul Smock","orcid":null,"position":5,"is_corresponding":false},{"id":863778,"name":"Jocelyn Jakubik","orcid":null,"position":6,"is_corresponding":false},{"id":229219,"name":"Yenny Goez-Gazi","orcid":null,"position":7,"is_corresponding":false},{"id":229222,"name":"Michal Gaži","orcid":null,"position":8,"is_corresponding":false},{"id":225361,"name":"John Dutton","orcid":"0000-0001-6253-2342","position":9,"is_corresponding":false},{"id":225362,"name":"Elizabeth A. Clemmons","orcid":"0000-0002-2403-9415","position":10,"is_corresponding":false},{"id":918995,"name":"Marc E. Mattix","orcid":null,"position":11,"is_corresponding":false},{"id":225367,"name":"Ricardo Carrion","orcid":"0000-0001-7046-7683","position":12,"is_corresponding":false},{"id":736448,"name":"Thomas L. Rudge","orcid":"0000-0002-8801-8744","position":13,"is_corresponding":false},{"id":918996,"name":"Alex Ridenour","orcid":null,"position":14,"is_corresponding":false},{"id":918997,"name":"Sovann F. Woodin","orcid":null,"position":15,"is_corresponding":false},{"id":383716,"name":"Ruth Hunegnaw","orcid":"0000-0002-4212-5245","position":16,"is_corresponding":false},{"id":266889,"name":"Nancy J. Sullivan","orcid":"0000-0002-2159-2039","position":17,"is_corresponding":false},{"id":918443,"name":"Rong Xu","orcid":"0000-0003-2976-1914","position":18,"is_corresponding":false},{"id":218417,"name":"Courtney L. Finch","orcid":"0000-0002-3674-3282","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:26:25.074177Z","pmid":"36423030","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}