{"doi":"10.3390/v17081095","title":"Homologous and Heterologous Vaccination Regimens with mRNA and rVSV Platforms Induce Potent Immune Responses Against SFTSV Glycoprotein","abstract":"BACKGROUND: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic bunyavirus with a high case-fatality ratio for which there is no approved vaccine. Studies have assessed different vaccine technologies. However, few studies have yet assessed the immunogenicity of heterologous prime-boost regimens. METHODS: Here, we compare a lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA-based vaccine encoding the SFTSV glycoproteins, Gn and Gc, to our recently described recombinant VSV SFTSV (rVSV-SFTSV) vaccine in single dose, homologous, and heterologous prime-boost regimens in mice. RESULTS: We show that all regimens protect from pathogenic SFTSV challenge and elicit strong long-lasting antibody responses. Furthermore, strong cellular immunity is elicited by mRNA-LNP immunizations and by heterologous immunization with an rVSV-SFTSV prime and mRNA-LNP boost. Cellular responses robustly polarized towards a type 1 response, characterized by high levels of IFNγ, TNFα, and IL-2. Immunization with mRNA led to a mixed type 1/type 2 immune response, as determined by antibody isotypes IgG1 and IgG2c. We found that homologous immunization leads to stronger antibody responses while heterologous immunization drives a slightly stronger cellular response. CONCLUSIONS: Taken together, the vaccine platforms described here represent strong vaccine candidates for further development.","journal":"Viruses","year":2025,"id":517596,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9653,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":294448,"name":"Jonna B. Westover","orcid":"0000-0002-3642-2980","position":1,"is_corresponding":false},{"id":230177,"name":"Kendall A. Lundgreen","orcid":"0000-0003-2431-7152","position":2,"is_corresponding":false},{"id":230380,"name":"Philip Hicks","orcid":"0000-0002-1574-5692","position":3,"is_corresponding":false},{"id":813580,"name":"Raegan J. Petch","orcid":"0000-0002-4508-4362","position":4,"is_corresponding":false},{"id":812113,"name":"Jordan T. Ort","orcid":"0000-0001-6977-4168","position":5,"is_corresponding":false},{"id":20753,"name":"Drew Weissman","orcid":"0000-0002-1501-6510","position":6,"is_corresponding":false},{"id":233975,"name":"Steven H.Y. Fan","orcid":null,"position":7,"is_corresponding":false},{"id":105476,"name":"Scott E. Hensley","orcid":"0000-0002-2928-7506","position":8,"is_corresponding":false},{"id":54904,"name":"Norbert Pardi","orcid":"0000-0003-1008-6242","position":9,"is_corresponding":false},{"id":294449,"name":"Brian B. Gowen","orcid":"0000-0001-9113-2575","position":10,"is_corresponding":false},{"id":230179,"name":"Paul Bates","orcid":"0000-0002-3918-5976","position":11,"is_corresponding":false},{"id":230175,"name":"Tomaz B. Manzoni","orcid":"0000-0001-6380-5103","position":0,"is_corresponding":true}],"reference_count":134,"raw_metadata":null,"created_at":"2026-07-19T02:48:59.410472Z","pmid":"40872809","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}