{"doi":"10.3390/v16040592","title":"High Prevalence of Hepatitis B Virus Drug Resistance Mutations to Lamivudine among People with HIV/HBV Coinfection in Rural and Peri-Urban Communities in Botswana","abstract":"(1) Background: We aimed to determine the prevalence of hepatitis B virus (HBV) resistance-associated mutations (RAMs) in people with HBV and human immunodeficiency virus (HBV/HIV) in Botswana. (2) Methods: We sequenced HBV deoxyribonucleic acid (DNA) from participants with HBV/HIV from the Botswana Combination Prevention Project study (2013-2018) using the Oxford Nanopore GridION platform. Consensus sequences were analyzed for genotypic and mutational profiles. (3) Results: Overall, 98 HBV sequences had evaluable reverse transcriptase region coverage. The median participant age was 43 years (IQR: 37, 49) and 66/98 (67.4%) were female. Most participants, i.e., 86/98 (87.8%) had suppressed HIV viral load (VL). HBV RAMs were identified in 61/98 (62.2%) participants. Most RAMs were in positions 204 (60.3%), 180 (50.5%), and 173 (33.3%), mostly associated with lamivudine resistance. The triple mutations rtM204V/L180M/V173L were the most predominant (17/61 [27.9%]). Most participants (96.7%) with RAMs were on antiretroviral therapy for a median duration of 7.5 years (IQR: 4.8, 10.5). Approximately 27.9% (17/61) of participants with RAMs had undetectable HBV VL, 50.8% (31/61) had VL < 2000 IU/mL, and 13/61 (21.3%) had VL ≥ 2000 IU/mL. (4) Conclusions: The high prevalence of lamivudine RAMs discourages the use of ART regimens with 3TC as the only HBV-active drug in people with HIV/HBV.","journal":"Viruses","year":2024,"id":440118,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9232,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":425976,"name":"Motswedi Anderson","orcid":"0000-0001-9974-9684","position":1,"is_corresponding":false},{"id":940997,"name":"Irene Gobe","orcid":"0000-0003-3679-6509","position":2,"is_corresponding":false},{"id":828773,"name":"Margaret Mokomane","orcid":"0000-0002-4406-3594","position":3,"is_corresponding":false},{"id":425978,"name":"Wonderful T. Choga","orcid":"0000-0001-7606-0569","position":4,"is_corresponding":false},{"id":1096091,"name":"Basetsana Phakedi","orcid":"0009-0005-9029-6427","position":5,"is_corresponding":false},{"id":1096090,"name":"Tsholofelo Ratsoma","orcid":"0009-0006-9815-9088","position":6,"is_corresponding":false},{"id":1068192,"name":"Gorata Mpebe","orcid":null,"position":7,"is_corresponding":false},{"id":393322,"name":"Joseph Makhema","orcid":"0000-0003-0017-2438","position":8,"is_corresponding":false},{"id":431953,"name":"Roger Shapiro","orcid":"0000-0002-4832-6275","position":9,"is_corresponding":false},{"id":257497,"name":"Shahin Lockman","orcid":"0000-0002-5384-9716","position":10,"is_corresponding":false},{"id":425980,"name":"Rosemary Musonda","orcid":"0009-0009-9937-6908","position":11,"is_corresponding":false},{"id":425983,"name":"Sikhulile Moyo","orcid":"0000-0003-3821-4592","position":12,"is_corresponding":false},{"id":425985,"name":"Simani Gaseitsiwe","orcid":"0000-0002-7089-3735","position":13,"is_corresponding":false},{"id":425975,"name":"Bonolo B. Phinius","orcid":"0000-0002-0180-2705","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":null,"created_at":"2026-07-19T02:00:57.233748Z","pmid":"38675933","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}