{"doi":"10.3390/v15051077","title":"A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses","abstract":"The henipaviruses, Nipah virus (NiV), and Hendra virus (HeV) can cause fatal diseases in humans and animals, whereas Cedar virus is a nonpathogenic henipavirus. Here, using a recombinant Cedar virus (rCedV) reverse genetics platform, the fusion (F) and attachment (G) glycoprotein genes of rCedV were replaced with those of NiV-Bangladesh (NiV-B) or HeV, generating replication-competent chimeric viruses (rCedV-NiV-B and rCedV-HeV), both with and without green fluorescent protein (GFP) or luciferase protein genes. The rCedV chimeras induced a Type I interferon response and utilized only ephrin-B2 and ephrin-B3 as entry receptors compared to rCedV. The neutralizing potencies of well-characterized cross-reactive NiV/HeV F and G specific monoclonal antibodies against rCedV-NiV-B-GFP and rCedV-HeV-GFP highly correlated with measurements obtained using authentic NiV-B and HeV when tested in parallel by plaque reduction neutralization tests (PRNT). A rapid, high-throughput, and quantitative fluorescence reduction neutralization test (FRNT) using the GFP-encoding chimeras was established, and monoclonal antibody neutralization data derived by FRNT highly correlated with data derived by PRNT. The FRNT assay could also measure serum neutralization titers from henipavirus G glycoprotein immunized animals. These rCedV chimeras are an authentic henipavirus-based surrogate neutralization assay that is rapid, cost-effective, and can be utilized outside high containment.","journal":"Viruses","year":2023,"id":346007,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9581,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1054972,"name":"Randy Yin","orcid":"0000-0002-3462-6736","position":1,"is_corresponding":false},{"id":551665,"name":"Lianying Yan","orcid":"0000-0003-1663-1205","position":2,"is_corresponding":false},{"id":124945,"name":"Viktoriya Borisevich","orcid":null,"position":3,"is_corresponding":false},{"id":802757,"name":"Bishwo N. Adhikari","orcid":"0000-0003-4840-9609","position":4,"is_corresponding":false},{"id":638835,"name":"Andrew J. Bennett","orcid":"0000-0003-1032-4781","position":5,"is_corresponding":false},{"id":239770,"name":"Francisco Malagón","orcid":"0000-0002-7041-0780","position":6,"is_corresponding":false},{"id":802760,"name":"Regina Z. Cer","orcid":"0000-0002-2395-980X","position":7,"is_corresponding":false},{"id":802761,"name":"Kimberly A. Bishop‐Lilly","orcid":"0000-0002-5744-8493","position":8,"is_corresponding":false},{"id":638436,"name":"Antony S. Dimitrov","orcid":"0000-0001-8356-6701","position":9,"is_corresponding":false},{"id":242853,"name":"Robert W. Cross","orcid":"0000-0001-7718-1522","position":10,"is_corresponding":false},{"id":242852,"name":"Thomas W. Geisbert","orcid":"0000-0003-0858-1877","position":11,"is_corresponding":false},{"id":533697,"name":"Christopher C. Broder","orcid":"0000-0001-5382-0510","position":12,"is_corresponding":false},{"id":646028,"name":"Moushimi Amaya","orcid":"0000-0002-7186-9673","position":0,"is_corresponding":true}],"reference_count":82,"raw_metadata":null,"created_at":"2026-07-19T01:11:44.152490Z","pmid":"37243163","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}