{"doi":"10.3390/v14092069","title":"Autopsy Study Defines Composition and Dynamics of the HIV-1 Reservoir after Allogeneic Hematopoietic Stem Cell Transplantation with CCR5Δ32/Δ32 Donor Cells","abstract":"Allo-HSCT with CCR5Δ32/Δ32 donor cells is the only curative HIV-1 intervention. We investigated the impact of allo-HSCT on the viral reservoir in PBMCs and post-mortem tissue in two patients. IciS-05 and IciS-11 both received a CCR5Δ32/Δ32 allo-HSCT. Before allo-HSCT, ultrasensitive HIV-1 RNA quantification; HIV-1-DNA quantification; co-receptor tropism analysis; deep-sequencing and viral characterization in PBMCs and bone marrow; and post-allo-HSCT, ultrasensitive RNA and HIV-1-DNA quantification were performed. Proviral quantification, deep sequencing, and viral characterization were done in post-mortem tissue samples. Both patients harbored subtype B CCR5-tropic HIV-1 as determined genotypically and functionally by virus culture. Pre-allo-HSCT, HIV-1-DNA could be detected in both patients in bone marrow, PBMCs, and T-cell subsets. Chimerism correlated with detectable HIV-1-DNA LTR copies in cells and tissues. Post-mortem analysis of IciS-05 revealed proviral DNA in all tissue biopsies, but not in PBMCs. In patient IciS-11, who was transplanted twice, no HIV-1-DNA could be detected in PBMCs at the time of death, whereas HIV-1-DNA was detectable in the lymph node. In conclusion, shortly after CCR5Δ32/Δ32, allo-HSCT HIV-1-DNA became undetectable in PBMCs. However, HIV-1-DNA variants identical to those present before transplantation persisted in post-mortem-obtained tissues, indicating that these tissues play an important role as viral reservoirs.","journal":"Viruses","year":2022,"id":273186,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9565,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":940214,"name":"Anke H. W. Bruns","orcid":"0000-0002-2420-2067","position":1,"is_corresponding":false},{"id":940215,"name":"A. Stam","orcid":"0000-0003-2038-6791","position":2,"is_corresponding":false},{"id":940679,"name":"Pauline M. Ellerbroek","orcid":null,"position":3,"is_corresponding":false},{"id":940680,"name":"Dorien de Jong","orcid":null,"position":4,"is_corresponding":false},{"id":940681,"name":"Noémi A. Nagy","orcid":null,"position":5,"is_corresponding":false},{"id":511482,"name":"Stephanie B. H. Gumbs","orcid":"0000-0003-0797-437X","position":6,"is_corresponding":false},{"id":940216,"name":"Kiki Tesselaar","orcid":"0000-0002-9847-0814","position":7,"is_corresponding":false},{"id":940217,"name":"Kobus J. Bosman","orcid":"0000-0001-5973-5130","position":8,"is_corresponding":false},{"id":542893,"name":"María Salgado","orcid":"0000-0003-1551-7216","position":9,"is_corresponding":false},{"id":940682,"name":"Gero Hütter","orcid":null,"position":10,"is_corresponding":false},{"id":205276,"name":"Lodewijk A.A. Brosens","orcid":"0000-0003-1341-8994","position":11,"is_corresponding":false},{"id":940218,"name":"Mi Kwon","orcid":"0000-0002-3855-7774","position":12,"is_corresponding":false},{"id":940683,"name":"José Diez Martin","orcid":null,"position":13,"is_corresponding":false},{"id":940684,"name":"Jan T. M. van der Meer","orcid":null,"position":14,"is_corresponding":false},{"id":940219,"name":"Theun M. de Kort","orcid":"0000-0002-5266-8120","position":15,"is_corresponding":false},{"id":369706,"name":"Asier Sáez‐Cirión","orcid":"0000-0003-2406-7536","position":16,"is_corresponding":false},{"id":449503,"name":"Julian Schulze zur Wiesch","orcid":"0000-0002-5033-1938","position":17,"is_corresponding":false},{"id":297903,"name":"Jaap Jan Boelens","orcid":"0000-0003-2232-6952","position":18,"is_corresponding":false},{"id":31314,"name":"Javier Martínez‐Picado","orcid":"0000-0002-4916-2129","position":19,"is_corresponding":false},{"id":428642,"name":"Jürgen Kuball","orcid":"0000-0002-3914-7806","position":20,"is_corresponding":false},{"id":69686,"name":"Annemarie M. J. Wensing","orcid":"0000-0003-3790-8891","position":21,"is_corresponding":false},{"id":940220,"name":"Monique Nijhuis","orcid":"0000-0001-6610-8282","position":22,"is_corresponding":false},{"id":940678,"name":"Laura E. P. Huyveneers","orcid":null,"position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-19T00:27:52.048702Z","pmid":"36146874","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}