{"doi":"10.3390/v14030611","title":"A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins","abstract":"The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here, the cellular impact of expressing SARS-CoV-2 viral proteins was studied by global proteomic analysis, and proximity biotinylation (BioID) was used to map the SARS-CoV-2 virus-host interactome in human lung cancer-derived cells. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are associated with SARS-CoV-2 proteins. We have established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Furthermore, we identified 66 high-confidence interactions by comparing this study with previous reports, providing a strong foundation for future follow-up studies. Finally, we cross-referenced candidate interactors with the CLUE drug library to identify potential therapeutics for drug-repurposing efforts. Collectively, these studies provide a valuable resource to uncover novel SARS-CoV-2 biology and inform development of antivirals.","journal":"Viruses","year":2022,"id":239445,"datarank":0.586803450814222,"base_score":3.912023005428146,"endowment":3.912023005428146,"self_citation_contribution":0.586803450814222,"citation_network_contribution":0.0,"self_endowment_contribution":0.586803450814222,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":107909,"name":"Laura Martin‐Sancho","orcid":"0000-0003-1489-2815","position":1,"is_corresponding":false},{"id":807859,"name":"Valesca Anschau","orcid":"0000-0002-9681-7204","position":2,"is_corresponding":false},{"id":621844,"name":"Sophie Liu","orcid":"0000-0001-7934-8605","position":3,"is_corresponding":false},{"id":808476,"name":"Rachel J. Chrisopulos","orcid":null,"position":4,"is_corresponding":false},{"id":245514,"name":"Kelsey L. Scott","orcid":"0000-0003-1378-5348","position":5,"is_corresponding":false},{"id":708028,"name":"Charles T. Halfmann","orcid":"0000-0001-8972-3560","position":6,"is_corresponding":false},{"id":808477,"name":"Ramon Díaz Peña","orcid":null,"position":7,"is_corresponding":false},{"id":342870,"name":"Dexter Pratt","orcid":"0000-0002-1471-9513","position":8,"is_corresponding":false},{"id":259808,"name":"Alexandre Rosa Campos","orcid":"0000-0003-3988-7764","position":9,"is_corresponding":false},{"id":245516,"name":"Kyle J. Roux","orcid":"0000-0001-7530-5528","position":10,"is_corresponding":false},{"id":245513,"name":"Danielle G. May","orcid":"0000-0002-2628-4414","position":0,"is_corresponding":true}],"reference_count":97,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:22:36.865157Z","pmid":"35337019","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}