{"doi":"10.3390/v13122353","title":"Analysis of Hendra Virus Fusion Protein N-Terminal Transmembrane Residues","abstract":"Hendra virus (HeV) is a zoonotic enveloped member of the family Paramyoxviridae. To successfully infect a host cell, HeV utilizes two surface glycoproteins: the attachment (G) protein to bind, and the trimeric fusion (F) protein to merge the viral envelope with the membrane of the host cell. The transmembrane (TM) region of HeV F has been shown to have roles in F protein stability and the overall trimeric association of F. Previously, alanine scanning mutagenesis has been performed on the C-terminal end of the protein, revealing the importance of β-branched residues in this region. Additionally, residues S490 and Y498 have been demonstrated to be important for F protein endocytosis, needed for the proteolytic processing of F required for fusion. To complete the analysis of the HeV F TM, we performed alanine scanning mutagenesis to explore the residues in the N-terminus of this region (residues 487-506). In addition to confirming the critical roles for S490 and Y498, we demonstrate that mutations at residues M491 and L492 alter F protein function, suggesting a role for these residues in the fusion process.","journal":"Viruses","year":2021,"id":205440,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":706675,"name":"Hadley E. Neal","orcid":"0000-0001-8433-593X","position":1,"is_corresponding":false},{"id":707174,"name":"Kearstin Edmonds","orcid":null,"position":2,"is_corresponding":false},{"id":710119,"name":"J. Lizbeth Reyes Zamora","orcid":"0000-0003-3000-817X","position":3,"is_corresponding":false},{"id":707175,"name":"Carole L. Moncman","orcid":null,"position":4,"is_corresponding":false},{"id":788760,"name":"Andreea Popa","orcid":"0009-0008-0422-1325","position":5,"is_corresponding":false},{"id":607945,"name":"Everett Clinton Smith","orcid":"0000-0002-3221-2476","position":6,"is_corresponding":false},{"id":789206,"name":"Stacy R. Webb","orcid":null,"position":7,"is_corresponding":false},{"id":300660,"name":"Rebecca Dutch","orcid":"0000-0002-5220-8247","position":8,"is_corresponding":false},{"id":303005,"name":"Chelsea T. Barrett","orcid":null,"position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-18T23:51:33.996102Z","pmid":"34960622","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}