{"doi":"10.3390/v13112115","title":"Binding of a Pocket Factor to Hepatitis B Virus Capsids Changes the Rotamer Conformation of Phenylalanine 97","abstract":"(1) Background: During maturation of the Hepatitis B virus, a viral polymerase inside the capsid transcribes a pre-genomic RNA into a partly double stranded DNA-genome. This is followed by envelopment with surface proteins inserted into a membrane. Envelopment is hypothetically regulated by a structural signal that reports the maturation state of the genome. NMR data suggest that such a signal can be mimicked by the binding of the detergent Triton X 100 to hydrophobic pockets in the capsid spikes. (2) Methods: We have used electron cryo-microscopy and image processing to elucidate the structural changes that are concomitant with the binding of Triton X 100. (3) Results: Our maps show that Triton X 100 binds with its hydrophobic head group inside the pocket. The hydrophilic tail delineates the outside of the spike and is coordinated via Lys-96. The binding of Triton X 100 changes the rotamer conformation of Phe-97 in helix 4, which enables a π-stacking interaction with Trp-62 in helix 3. Similar changes occur in mutants with low secretion phenotypes (P5T and L60V) and in a mutant with a pre-mature secretion phenotype (F97L). (4) Conclusion: Binding of Triton X 100 is unlikely to mimic structural maturation because mutants with different secretion phenotypes show similar structural responses.","journal":"Viruses","year":2021,"id":198035,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9589,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":772604,"name":"Christian Kraft","orcid":"0000-0002-6483-4296","position":1,"is_corresponding":false},{"id":772605,"name":"Matthias Grießmann","orcid":"0000-0001-9883-8415","position":2,"is_corresponding":false},{"id":772606,"name":"Tim Rasmussen","orcid":"0000-0003-1126-846X","position":3,"is_corresponding":false},{"id":773146,"name":"Kilian Katzenberger","orcid":null,"position":4,"is_corresponding":false},{"id":773147,"name":"Melina Lappe","orcid":null,"position":5,"is_corresponding":false},{"id":773148,"name":"Paul Pfarr","orcid":null,"position":6,"is_corresponding":false},{"id":773149,"name":"Cato Stoffer","orcid":null,"position":7,"is_corresponding":false},{"id":773150,"name":"Mara Stöhr","orcid":null,"position":8,"is_corresponding":false},{"id":773151,"name":"Anna-Maria Wandinger","orcid":null,"position":9,"is_corresponding":false},{"id":685328,"name":"Bettina Böttcher","orcid":"0000-0002-7962-4849","position":10,"is_corresponding":false},{"id":685339,"name":"Cihan Makbul","orcid":"0000-0002-5611-3456","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-18T23:50:27.616568Z","pmid":"34834922","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}