{"doi":"10.3390/v13050753","title":"Stem Cell-Derived Viral Antigen-Specific T Cells Suppress HIV Replication and PD-1 Expression on CD4+ T Cells","abstract":"The viral antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) derived from pluripotent stem cells (PSCs), i.e., PSC-CTLs, have the ability to suppress the human immunodeficiency virus (HIV) infection. After adoptive transfer, PSC-CTLs can infiltrate into the local tissues to suppress HIV replication. Nevertheless, the mechanisms by which the viral Ag-specific PSC-CTLs elicit the antiviral response remain to be fully elucidated. In this study, we generated the functional HIV-1 Gag epitope SL9-specific CTLs from the induced PSC (iPSCs), i.e., iPSC-CTLs, and investigated the suppression of SL9-specific iPSC-CTLs on viral replication and the protection of CD4+ T cells. A chimeric HIV-1, i.e., EcoHIV, was used to produce HIV replication in mice. We show that adoptive transfer of SL9-specific iPSC-CTLs greatly suppressed EcoHIV replication in the peritoneal macrophages and spleen in the animal model. Furthermore, we demonstrate that the adoptive transfer significantly reduced expression of PD-1 on CD4+ T cells in the spleen and generated persistent anti-HIV memory T cells. These results indicate that stem cell-derived viral Ag-specific CTLs can robustly accumulate in the local tissues to suppress HIV replication and prevent CD4+ T cell exhaustion through reduction of PD-1 expression.","journal":"Viruses","year":2021,"id":195467,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9484,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":517658,"name":"Fengyang Lei","orcid":"0000-0002-9169-8314","position":1,"is_corresponding":false},{"id":486874,"name":"Xiaofang Xiong","orcid":"0000-0001-8276-4415","position":2,"is_corresponding":false},{"id":486873,"name":"Yijie Ren","orcid":"0000-0002-5753-421X","position":3,"is_corresponding":false},{"id":486872,"name":"Hao‐Yun Peng","orcid":"0000-0001-5151-590X","position":4,"is_corresponding":false},{"id":766761,"name":"Liqing Wang","orcid":"0000-0001-8769-5148","position":5,"is_corresponding":false},{"id":486871,"name":"Anil Kumar","orcid":"0000-0002-7611-5918","position":6,"is_corresponding":false},{"id":486870,"name":"Jugal Kishore Das","orcid":"0000-0002-4553-2196","position":7,"is_corresponding":false},{"id":486875,"name":"Jianxun Song","orcid":"0000-0002-9734-6176","position":8,"is_corresponding":false},{"id":517657,"name":"Mohammad Haque","orcid":"0000-0002-1350-0595","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-18T23:50:07.691929Z","pmid":"33923025","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}