{"doi":"10.3390/v12121346","title":"Structural Basis of Zika Virus Specific Neutralization in Subsequent Flavivirus Infections","abstract":"Zika virus (ZIKV), a mosquito-borne human flavivirus that causes microcephaly and other neurological disorders, has been a recent focus for the development of flavivirus vaccines and therapeutics. We report here a 4.0 Å resolution structure of the mature ZIKV in complex with ADI-30056, a ZIKV-specific human monoclonal antibody (hMAb) isolated from a ZIKV infected donor with a prior dengue virus infection. The structure shows that the hMAb interactions span across the E protein dimers on the virus surface, inhibiting conformational changes required for the formation of infectious fusogenic trimers similar to the hMAb, ZIKV-117. Structure-based functional analysis, and structure and sequence comparisons, identified ZIKV residues essential for neutralization and crucial for the evolution of highly potent E protein crosslinking Abs in ZIKV. Thus, this epitope, ZIKV's \"Achilles heel\", defined by the contacts between ZIKV and ADI-30056, could be a suitable target for the design of therapeutic antibodies.","journal":"Viruses","year":2020,"id":83157,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9421,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":108976,"name":"Thomas F. Rogers","orcid":"0000-0001-9756-327X","position":1,"is_corresponding":false},{"id":314292,"name":"Andrew S. Miller","orcid":"0000-0002-1366-184X","position":2,"is_corresponding":false},{"id":428252,"name":"Feng Long","orcid":"0000-0002-9625-0527","position":3,"is_corresponding":false},{"id":327015,"name":"Thomas Klose","orcid":"0000-0002-2150-9792","position":4,"is_corresponding":false},{"id":428253,"name":"Nathan Beutler","orcid":"0000-0003-3240-9524","position":5,"is_corresponding":false},{"id":428254,"name":"Yen‐Chung Lai","orcid":"0000-0002-3844-3080","position":6,"is_corresponding":false},{"id":428255,"name":"Mara Parren","orcid":"0000-0003-0067-0154","position":7,"is_corresponding":false},{"id":109550,"name":"Laura M. Walker","orcid":"0000-0001-7704-3197","position":8,"is_corresponding":false},{"id":429285,"name":"Geeta Buda","orcid":null,"position":9,"is_corresponding":false},{"id":108980,"name":"Dennis R. Burton","orcid":"0000-0001-6711-9864","position":10,"is_corresponding":false},{"id":91658,"name":"Michael G. Rossmann","orcid":"0000-0002-9468-9309","position":11,"is_corresponding":false},{"id":314297,"name":"Richard Kühn","orcid":"0000-0003-4148-1026","position":12,"is_corresponding":false},{"id":428251,"name":"Madhumati Sevvana","orcid":"0000-0003-4992-6296","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-18T21:54:09.383533Z","pmid":"33255202","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}