{"doi":"10.3390/s25175262","title":"Development of a Miniaturized, Automated, and Cost-Effective Device for Enzyme-Linked Immunosorbent Assay","abstract":"In this work, a miniaturized, automated, and cost-effective ELISA device is designed and implemented, without the utilization of conventional techniques such as pipetting or microfluidic valve technologies. The device has dimensions of 24 cm × 19 cm × 14 cm and weighs <3 kg. The total hardware cost of the device is estimated to be approximately $1200, which can be further reduced through optimization during scale-up production. Three-dimensional printed disposable parts, including the reagent reservoir disk and the microfluidic connector, have also been developed. IL-6 is used as a model system to demonstrate how the device provides an ELISA measurement. The cost per test is estimated to be <$10. The compactness, automated operation, along with the cost-effectiveness of this ELISA device, makes it suitable for point-of-care applications in resource-limited regions.","journal":"Sensors","year":2025,"id":546346,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9529,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1438072,"name":"Shuo Yang","orcid":"0000-0003-3351-5249","position":1,"is_corresponding":false},{"id":1438471,"name":"Suchithra Guntur","orcid":null,"position":2,"is_corresponding":false},{"id":1438472,"name":"Vaishnavi Potluri","orcid":null,"position":3,"is_corresponding":false},{"id":1438073,"name":"Girish S. Kulkarni","orcid":"0000-0003-0125-0856","position":4,"is_corresponding":false},{"id":406296,"name":"Xudong Fan","orcid":"0000-0003-0149-1326","position":5,"is_corresponding":false},{"id":1438071,"name":"Majid Aalizadeh","orcid":"0000-0002-4312-1940","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":null,"created_at":"2026-07-19T02:53:32.285899Z","pmid":"40942690","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}