{"doi":"10.3390/plants11243527","title":"TPC1-Type Channels in Physcomitrium patens: Interaction between EF-Hands and Ca2+","abstract":"Two-pore channels (TPCs) are members of the superfamily of ligand-gated and voltage-sensitive ion channels in the membranes of intracellular organelles of eukaryotic cells. The evolution of ordinary plant TPC1 essentially followed a very conservative pattern, with no changes in the characteristic structural footprints of these channels, such as the cytosolic and luminal regions involved in Ca2+ sensing. In contrast, the genomes of mosses and liverworts encode also TPC1-like channels with larger variations at these sites (TPC1b channels). In the genome of the model plant Physcomitrium patens we identified nine non-redundant sequences belonging to the TPC1 channel family, two ordinary TPC1-type, and seven TPC1b-type channels. The latter show variations in critical amino acids in their EF-hands essential for Ca2+ sensing. To investigate the impact of these differences between TPC1 and TPC1b channels, we generated structural models of the EF-hands of PpTPC1 and PpTPC1b channels. These models were used in molecular dynamics simulations to determine the frequency with which calcium ions were present in a coordination site and also to estimate the average distance of the ions from the center of this site. Our analyses indicate that the EF-hand domains of PpTPC1b-type channels have a lower capacity to coordinate calcium ions compared with those of common TPC1-like channels.","journal":"Plants","year":2022,"id":287934,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9287,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":968373,"name":"Fernando Vergara-Valladares","orcid":"0000-0003-4957-3427","position":1,"is_corresponding":false},{"id":968374,"name":"María Rubio-Meléndez","orcid":"0000-0002-9852-427X","position":2,"is_corresponding":false},{"id":968375,"name":"Naomí Hernández-Rojas","orcid":"0000-0002-1400-5449","position":3,"is_corresponding":false},{"id":968376,"name":"Angélica González‐González","orcid":"0000-0001-7377-6545","position":4,"is_corresponding":false},{"id":258079,"name":"Erwan Michard","orcid":"0000-0001-7997-3822","position":5,"is_corresponding":false},{"id":968377,"name":"Carlos Navarro‐Retamal","orcid":"0000-0001-9581-0206","position":6,"is_corresponding":false},{"id":910063,"name":"Ingo Drèyer","orcid":"0000-0002-2781-0359","position":7,"is_corresponding":false},{"id":968863,"name":"Franko Mérida-Quesada","orcid":null,"position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-19T00:30:11.069895Z","pmid":"36559639","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}