{"doi":"10.3390/pharmaceutics17101273","title":"Leveraging the Immune Response from LIFE Biomaterial and Photon-Flash in Pre-Clinical Pancreatic Cancer Treatment","abstract":"Pre-clinical animal studies evaluating the ‘flash effect’ caused by ultra-high dose rate (≥40 Gy/s) favorably spares normal tissue from radiation-caused toxicity while maintaining anti-tumor effects like conventional (CONV) radiation. The goal of this study is to leverage an immune response resulting from the treatment combination of flash radiotherapy (Flash-RT) and LIFE (liquid immunogenic fiducial eluter) biomaterial incorporating an anti-mouse CD40 monoclonal antibody to enhance the therapeutic ratio in pancreatic cancer. Methods: A small animal FLASH radiation research platform (FLASH-SARRP) was utilized to deliver both ultra-high and CONV dose-rate irradiation to treat syngeneic subcutaneous pancreatic tumors generated in 8–10-week-old male and female C57BL6 mice. The efficacy of FLASH versus CONV radiotherapy (RT) at varying doses of 5, 8, 10, and 15 Gy delivered in a single fraction was evaluated by assessing tumor growth and mice survival over time or comparing tumor weight at 10 days post-treatment. Results: Similar tumor control capability was observed by the high-dose rate and conventional RT related to the control group. Nevertheless, longer survival was observed for the FLASH group at 5 Gy compared to CONV and control at either 5 Gy, 10 Gy, or 15 Gy doses. Multiplex immunofluorescence and immunohistochemistry results showed higher T-cell infiltration within the combination of RT (either FLASH or CONV) and LIFE biomaterial-treated tumors compared to the control cohort. Conclusions: This animal study serves as an impetus for future studies leveraging the immune response using the combination of FLASH and LIFE Biomaterial to enhance the efficacy of pancreatic cancer treatment.","journal":"Pharmaceutics","year":2025,"id":534873,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.955,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1279344,"name":"Katelyn Kelly","orcid":null,"position":1,"is_corresponding":false},{"id":577082,"name":"Sufeng Mao","orcid":"0009-0007-8768-8520","position":2,"is_corresponding":false},{"id":1088621,"name":"Debarghya China","orcid":"0000-0001-9053-8119","position":3,"is_corresponding":false},{"id":1418502,"name":"Girmachew Wasihun","orcid":null,"position":4,"is_corresponding":false},{"id":1418503,"name":"Aditya Pandya","orcid":null,"position":5,"is_corresponding":false},{"id":1418007,"name":"Mohammad Ali Tajik‐Mansoury","orcid":"0000-0002-9540-2672","position":6,"is_corresponding":false},{"id":1069271,"name":"Daniel Sforza","orcid":null,"position":7,"is_corresponding":false},{"id":828368,"name":"Devin Miles","orcid":"0000-0001-6052-5202","position":8,"is_corresponding":false},{"id":407760,"name":"Amol Narang","orcid":"0000-0002-0876-4967","position":9,"is_corresponding":false},{"id":1068823,"name":"Mohammad Rezaee","orcid":"0000-0003-4607-9570","position":10,"is_corresponding":false},{"id":257638,"name":"Wilfred Ngwa","orcid":"0000-0001-8317-6850","position":11,"is_corresponding":false},{"id":407759,"name":"Kai Ding","orcid":"0000-0002-8202-0173","position":12,"is_corresponding":false},{"id":371214,"name":"Michèle Moreau","orcid":"0000-0002-6881-6300","position":0,"is_corresponding":true}],"reference_count":29,"raw_metadata":null,"created_at":"2026-07-19T02:51:52.019261Z","pmid":"41155910","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}