{"doi":"10.3390/pharmaceutics17081045","title":"Anti-Neuroinflammation Effect of Standardized Ethanol Extract of Leaves of Perilla frutescens var. acuta on Aβ-Induced Alzheimer’s Disease-like Mouse Model","abstract":"Background/Objectives: Perilla frutescens var. acuta Kudo, a member of the Lamiaceae family, has been previously reported to reduce neuroinflammation and potentially decrease Aβ plaque accumulation in 5XFAD mice. In this study, we aimed to evaluate the anti-neuroinflammatory potential of a standardized 60% ethanol extract of Perilla leaves (PE), optimized for commercial application. Methods: The inflammatory response was assessed in LPS-stimulated BV2 microglial cells, and the cognitive improvement was evaluated in an AD animal model induced by intracerebroventricular injection of Aβ. Results: Using LPS-stimulated BV2 microglial cells and an Aβ-injected ICR mouse model of Alzheimer’s disease, we found that PE significantly suppressed the LPS-induced production of nitric oxide and pro-inflammatory mediators, including IL-6, TNF-α, NF-κB, iNOS, and COX-2, along with inhibition of JNK and p38 MAPK activation. Furthermore, PE upregulated CREB and BDNF expression. In vivo, PE administration alleviated Aβ-induced cognitive deficits, which were associated with reduced expression of JNK, NF-κB, iNOS, and COX and increased CREB/BDNF signaling in the hippocampus. Behavioral assessments—including passive avoidance, Morris water maze, novel object recognition, and Y-maze tests—confirmed the improvement in cognitive function. Conclusions: Collectively, these findings demonstrate that PE exerts significant anti-neuroinflammatory and neuroprotective effects, supporting its potential as a functional ingredient for cognitive enhancement.","journal":"Pharmaceutics","year":2025,"id":526552,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9564,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":849894,"name":"Jihye Lee","orcid":"0000-0002-1279-5037","position":1,"is_corresponding":false},{"id":1402665,"name":"Eunhong Lee","orcid":null,"position":2,"is_corresponding":false},{"id":1402666,"name":"Somin Moon","orcid":null,"position":3,"is_corresponding":false},{"id":1107815,"name":"Eunbi Cho","orcid":"0009-0009-0937-3958","position":4,"is_corresponding":false},{"id":1322136,"name":"Jieun Jeon","orcid":null,"position":5,"is_corresponding":false},{"id":1402667,"name":"A Young Park","orcid":null,"position":6,"is_corresponding":false},{"id":1402668,"name":"Joon‐Ho Hwang","orcid":null,"position":7,"is_corresponding":false},{"id":1402079,"name":"Gun Hee Cho","orcid":"0000-0001-8199-4278","position":8,"is_corresponding":false},{"id":1402669,"name":"Haram Kong","orcid":null,"position":9,"is_corresponding":false},{"id":1402080,"name":"Mi-Houn Park","orcid":"0000-0003-4834-5668","position":10,"is_corresponding":false},{"id":1402081,"name":"Sung‐Kyu Kim","orcid":"0000-0003-3597-748X","position":11,"is_corresponding":false},{"id":1402082,"name":"Dong Hyun Kim","orcid":"0000-0003-3394-2400","position":12,"is_corresponding":false},{"id":1402083,"name":"Ji Wook Jung","orcid":"0000-0002-1205-5696","position":13,"is_corresponding":false},{"id":1402664,"name":"Hyunji Kwon","orcid":null,"position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":null,"created_at":"2026-07-19T02:50:30.402772Z","pmid":"40871065","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}