{"doi":"10.3390/pharmaceutics14122750","title":"Topical Sustained Delivery of Miltefosine Via Drug-Eluting Contact Lenses to Treat Acanthamoeba Keratitis","abstract":"This study aimed to develop a miltefosine-eluting contact lens (MLF-CL) device that would allow sustained and localized miltefosine release for the treatment of Acanthamoeba keratitis. MLF-CLs were produced in three different miltefosine doses by solvent-casting a thin miltefosine-polymer film around the periphery of a methafilcon hydrogel, which was then lathed into a contact lens. During seven days of in vitro testing, all three formulations demonstrated sustained release from the lens at theoretically therapeutic levels. Based on the physicochemical characterization of MLF-CLs, MLF-CL's physical properties are not significantly different from commercial contact lenses in terms of light transmittance, water content and wettability. MLF-CLs possessed a slight reduction in compression modulus that was attributed to the inclusion of polymer-drug films but still remain within the optimal range of soft contact lenses. In cytotoxicity studies, MLF-CL indicated up to 91% viability, which decreased proportionally as miltefosine loading increased. A three-day biocompatibility test on New Zealand White rabbits revealed no impact of MLF-CLs on the corneal tissue. The MLF-CLs provided sustained in vitro release of miltefosine for a week while maintaining comparable physical features to a commercial contact lens. MLF-CL has a promising potential to be used as a successful treatment method for Acanthamoeba keratitis.","journal":"Pharmaceutics","year":2022,"id":266294,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":328062,"name":"Liangju Kuang","orcid":"0000-0003-1799-6716","position":1,"is_corresponding":false},{"id":742751,"name":"Amy E. Ross","orcid":null,"position":2,"is_corresponding":false},{"id":925975,"name":"Wissam Farhat","orcid":"0000-0002-1050-3723","position":3,"is_corresponding":false},{"id":925976,"name":"Nikolay Boychev","orcid":"0000-0003-2674-0835","position":4,"is_corresponding":false},{"id":926418,"name":"Sina Sharfi","orcid":null,"position":5,"is_corresponding":false},{"id":742139,"name":"Levi N. Kanu","orcid":"0000-0001-6428-4696","position":6,"is_corresponding":false},{"id":925977,"name":"Longqian Liu","orcid":"0000-0002-2265-4625","position":7,"is_corresponding":false},{"id":294091,"name":"Daniel S. Kohane","orcid":"0000-0001-5369-5932","position":8,"is_corresponding":false},{"id":325268,"name":"Joseph B. Ciolino","orcid":"0000-0002-6223-3091","position":9,"is_corresponding":false},{"id":925974,"name":"Lin Chen","orcid":"0000-0002-1187-2934","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-19T00:26:54.081997Z","pmid":"36559244","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}