{"doi":"10.3390/pathogens13020106","title":"The NLRP3 Inflammasome Is Dispensable in Methicillin-Resistant Staphylococcus aureus Urinary Tract Infection","abstract":"The NLRP3 inflammasome is a cytoplasmic complex that senses molecular patterns from pathogens or damaged cells to trigger an innate immune defense response marked by the production of proinflammatory cytokines IL-1β and IL-18 and an inflammatory death called pyroptosis. The NLRP3 inflammasome is activated in the urinary tract by a variety of infectious and non-infectious insults. In this study, we investigated the role of the NLRP3 inflammasome by comparing the pathophysiology of methicillin-resistant Staphylococcus aureus (MRSA) ascending UTI in wild-type (WT) and Nlrp3−/− mice. The difference in the bacterial burden detected in the urinary tracts of MRSA-infected WT and Nlrp3−/− was not statistically significant at 6, 24, and 72 h post-infection (hpi). The levels of pro-inflammatory cytokines and chemokines as well as the numbers of granulocytes recruited to bladder and kidney tissues at 24 hpi were also similar between Nlrp3−/− and WT mice. The histopathological analysis of MRSA-infected bladder and kidney sections from Nlrp3−/− and WT mice showed similar inflammation. Overall, these results suggest that MRSA-induced urinary NLRP3 activity does not play a role in the pathophysiology of the ascending UTI.","journal":"Pathogens","year":2024,"id":476244,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":740992,"name":"Rahul Kumar","orcid":"0000-0002-5677-3999","position":1,"is_corresponding":false},{"id":525702,"name":"Kenneth A. Rogers","orcid":"0000-0002-3911-035X","position":2,"is_corresponding":false},{"id":451666,"name":"Yogesh Saini","orcid":"0000-0002-8324-2122","position":3,"is_corresponding":false},{"id":451667,"name":"Sonika Patial","orcid":"0000-0002-7373-0835","position":4,"is_corresponding":false},{"id":879406,"name":"Ritwij Kulkarni","orcid":"0000-0002-9753-8899","position":5,"is_corresponding":false},{"id":1010525,"name":"Santosh Paudel","orcid":"0000-0002-5266-391X","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T02:06:25.428435Z","pmid":"38392844","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}