{"doi":"10.3390/nu17101677","title":"Dietary Supplementation of Edible Mushroom Phallus atrovolvatus Aqueous Extract Attenuates Brain Changes in the AppNL−G−F Mouse Model of Alzheimer’s Disease","abstract":"Background/Objectives: Alzheimer’s disease (AD) is a neurodegenerative disease characterized by progressive dementia and brain accumulation of Aβ-peptide-containing plaques, gliosis, neuroimmune changes, and neurofibrillary tangles. Mushroom polysaccharides have been previously reported to have anti-neuroinflammation activity through the gut–brain axis. This study aimed to evaluate whether a dietary intervention with Phallus atrovolvatus, a recently identified edible mushroom in Thailand, could have a benefit on gut health and alleviate AD-related changes. Methods: Male and female 6–8-month-old littermate wild-type control (C57BL/6J) and AppNL−G−F mice were randomly assigned to either a control diet or a diet supplemented with mushroom aqueous extract (MAE) for 8 weeks to quantify changes in body weight, intestine, immune cells, short chain fatty acids, brain cytokines, amyloid-β (Aβ) levels, gliosis, and memory. Results: MAE had no adverse effects on gut leakiness and increased pyruvate levels in serum. Splenocyte immune profiling revealed a significant increase in the frequency of IgM+, IA_IE+, and CD14+ cells in MAE-administered AppNL−G−Ffemale mice compared to their vehicle controls. AppNL−G−Fmale mice that received MAE showed a significant increase in the frequency of cytotoxic CD8 T cells within the cervical lymph nodes compared to their wild-type counterparts. Aβ deposition and gliosis were significantly reduced in the hippocampi of the MAE-supplemented AppNL−G−F groups. However, MAE feeding did not alter spatial recognition memory in either sex or genotype compared to their vehicle groups. Conclusions: Our findings demonstrated that the administration of P. atrovolvatus aqueous extract showed neuroprotective potential against AD-related changes in the brain with no adverse impact on gut health and memory.","journal":"Nutrients","year":2025,"id":544226,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9669,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1434463,"name":"Wasaporn Chanput","orcid":"0000-0003-4536-9912","position":1,"is_corresponding":false},{"id":1434816,"name":"Lalida Rojanathammanee","orcid":null,"position":2,"is_corresponding":false},{"id":273242,"name":"Svetlana A. Golovko","orcid":null,"position":3,"is_corresponding":false},{"id":672132,"name":"Drew R. Seeger","orcid":"0000-0002-9382-2753","position":4,"is_corresponding":false},{"id":272290,"name":"Mikhail Y. Golovko","orcid":"0000-0003-1749-3158","position":5,"is_corresponding":false},{"id":687876,"name":"Suba Nookala","orcid":"0000-0002-4632-265X","position":6,"is_corresponding":false},{"id":272292,"name":"Colin K. Combs","orcid":"0000-0001-8671-5880","position":7,"is_corresponding":false},{"id":1434815,"name":"Raweephorn Kaewsaen","orcid":null,"position":0,"is_corresponding":true}],"reference_count":60,"raw_metadata":null,"created_at":"2026-07-19T02:53:12.864581Z","pmid":"40431417","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}