{"doi":"10.3390/nu16172869","title":"Pollen-Food Allergy Syndrome: From Food Avoidance to Deciphering the Potential Cross-Reactivity between Pru p 3 and Ole e 7","abstract":"<jats:p>Background: Cross-reactivity between nonspecific lipid transfer proteins could cause anaphylaxis, further influencing food avoidance and nutrient deficiencies. The one affecting olive pollen (Ole e 7) and peach (Pru p 3) may underlie a variety of pollen-food syndromes, though a deep molecular analysis is necessary. Methods: Three Ole e 7-monosensitised patients (MON_OLE), three Pru p 3-monosensitised patients (MON_PRU) and three bisensitised patients (BI) were selected. For epitope mapping, both digested proteins were incubated with patient sera, and the captured IgE-bound peptides were characterised by LC-MS. Results: The analysis revealed two Ole e 7 epitopes and the three Pru p 3 epitopes previously described. Interestingly, the “KSALALVGNKV” Ole e 7 peptide was recognised by MON_OLE, BI and MON_PRU patients. Conversely, all patients recognised the “ISASTNCATVK” Pru p 3 peptide. Although complete sequence alignment between both proteins revealed 32.6% identity, local alignment considering seven residue fragments showed 50 and 57% identity when comparing “ISASTNCATVK” with Ole e 7 and “KSALALVGNKV” with Pru p 3. Conclusions: This study mapped sIgE-Ole e 7-binding epitopes, paving the way for more precise diagnostic tools. Assuming non-significant sequence similarity, structural homology and shared key residues may underlie the potential cross-reactivity between Ole e 7 and Pru p 3 nsLTPs.</jats:p>","journal":"Nutrients","year":2024,"id":621943,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1606304,"name":"Rocío Aguado","orcid":"0000-0002-7120-5631","position":1,"is_corresponding":false},{"id":1606305,"name":"Juan Molina","orcid":null,"position":2,"is_corresponding":false},{"id":1606306,"name":"Antonio Trujillo-Aguilera","orcid":null,"position":3,"is_corresponding":false},{"id":651207,"name":"Mayte Villalba","orcid":"0000-0002-0042-9953","position":4,"is_corresponding":false},{"id":1230540,"name":"Araceli Díaz‐Perales","orcid":"0000-0002-1093-3627","position":5,"is_corresponding":false},{"id":1606307,"name":"Carmen Oeo-Santos","orcid":null,"position":6,"is_corresponding":false},{"id":362078,"name":"Eduardo Chicano‐Gálvez","orcid":"0000-0002-9481-9628","position":7,"is_corresponding":false},{"id":1606308,"name":"Nadine Blanco","orcid":"0000-0002-7684-5099","position":8,"is_corresponding":false},{"id":1606309,"name":"Ana Navas","orcid":null,"position":9,"is_corresponding":false},{"id":1606310,"name":"Berta Ruiz-León","orcid":null,"position":10,"is_corresponding":false},{"id":1606311,"name":"Aurora Jurado","orcid":"0000-0001-9092-8758","position":11,"is_corresponding":false},{"id":1606303,"name":"Paula Álvarez","orcid":"0000-0002-3023-3230","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Pollen-Food Allergy Syndrome: From Food Avoidance to Deciphering the Potential Cross-Reactivity between Pru p 3 and Ole e 7","abstract":"<jats:p>Background: Cross-reactivity between nonspecific lipid transfer proteins could cause anaphylaxis, further influencing food avoidance and nutrient deficiencies. The one affecting olive pollen (Ole e 7) and peach (Pru p 3) may underlie a variety of pollen-food syndromes, though a deep molecular analysis is necessary. Methods: Three Ole e 7-monosensitised patients (MON_OLE), three Pru p 3-monosensitised patients (MON_PRU) and three bisensitised patients (BI) were selected. For epitope mapping, both digested proteins were incubated with patient sera, and the captured IgE-bound peptides were characterised by LC-MS. Results: The analysis revealed two Ole e 7 epitopes and the three Pru p 3 epitopes previously described. Interestingly, the “KSALALVGNKV” Ole e 7 peptide was recognised by MON_OLE, BI and MON_PRU patients. Conversely, all patients recognised the “ISASTNCATVK” Pru p 3 peptide. Although complete sequence alignment between both proteins revealed 32.6% identity, local alignment considering seven residue fragments showed 50 and 57% identity when comparing “ISASTNCATVK” with Ole e 7 and “KSALALVGNKV” with Pru p 3. Conclusions: This study mapped sIgE-Ole e 7-binding epitopes, paving the way for more precise diagnostic tools. Assuming non-significant sequence similarity, structural homology and shared key residues may underlie the potential cross-reactivity between Ole e 7 and Pru p 3 nsLTPs.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39275185","pmcid":"PMC11396898","openalex_id":"https://openalex.org/W4401917539","authors":[],"funders":[{"funder_name":"Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía","grant_id":"PI-0127-2019","title":null},{"funder_name":"Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía","grant_id":"BECA18:A25","title":null},{"funder_name":"Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía","grant_id":"BECA20:A2","title":null}],"total_grants":3,"fwci":0.5517,"citation_percentile":0.65524881,"influential_citations":0,"citation_trend":[{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2072-6643/16/17/2869/pdf?version=1724751599","host_type":"journal"},{"url":"https://www.mdpi.com/2072-6643/16/17/2869/pdf?version=1724751599","host_type":"publisher"},{"url":"https://www.mdpi.com/2072-6643/16/17/2869/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/nu16172869","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39275185","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11396898","host_type":"repository"},{"url":"http://hdl.handle.net/10261/368108","host_type":"repository"},{"url":"http://hdl.handle.net/10396/29047","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/nu16172869","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11396898/pdf/nutrients-16-02869.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11396898","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11396898?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Food Allergy and Anaphylaxis Research","Mast cells and histamine","Prion Diseases and Protein Misfolding","Humans","Cross Reactions","Antigens, Plant","Food Hypersensitivity","Pollen","Immunoglobulin E","Olea","Plant Proteins","Female","Male","Prunus persica","Epitope Mapping","Adult","Rhinitis, Allergic, Seasonal","Amino Acid Sequence","Epitopes","Allergens","Middle Aged","Carrier Proteins"],"mesh_terms":["Prunus persica","Adult","Allergens","Amino Acid Sequence","Epitopes","Carrier Proteins","Cross Reactions","Female","Food Hypersensitivity","Rhinitis, Allergic, Seasonal","Humans","Immunoglobulin E","Male","Middle Aged","Plant Proteins","Pollen","Epitope Mapping","Olea","Antigens, Plant"],"keywords":["Cross-reactivity","Food allergy","Pollen","Anaphylaxis","Allergy","Plant lipid transfer proteins","Oral allergy syndrome","Biology","Cross reactions","Immunology","Medicine","Food science","Chemistry","Botany","Biochemistry","Gene","Antigen","Epitope mapping","Pollen-food Syndrome","Ole E 7 Allergen","Pru P 3 Allergen","Nonspecific Lipid Transfer Proteins (Nsltps)"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"uniprot"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T17:05:00.937265Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}