{"doi":"10.3390/nu13093290","title":"Gut Microbiota Predicts Healthy Late-Life Aging in Male Mice","abstract":"Calorie restriction (CR) extends lifespan and retards age-related chronic diseases in most species. There is growing evidence that the gut microbiota has a pivotal role in host health and age-related pathological conditions. Yet, it is still unclear how CR and the gut microbiota are related to healthy aging. Here, we report findings from a small longitudinal study of male C57BL/6 mice maintained on either ad libitum or mild (15%) CR diets from 21 months of age and tracked until natural death. We demonstrate that CR results in a significantly reduced rate of increase in the frailty index (FI), a well-established indicator of aging. We observed significant alterations in diversity, as well as compositional patterns of the mouse gut microbiota during the aging process. Interrogating the FI-related microbial features using machine learning techniques, we show that gut microbial signatures from 21-month-old mice can predict the healthy aging of 30-month-old mice with reasonable accuracy. This study deepens our understanding of the links between CR, gut microbiota, and frailty in the aging process of mice.","journal":"Nutrients","year":2021,"id":172823,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9475,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":258545,"name":"Sarah J. Mitchell","orcid":"0000-0002-4027-3754","position":1,"is_corresponding":false},{"id":264658,"name":"Michael R. MacArthur","orcid":"0000-0003-3593-6995","position":2,"is_corresponding":false},{"id":218499,"name":"Alice E. Kane","orcid":"0000-0002-4303-0491","position":3,"is_corresponding":false},{"id":106661,"name":"David Sinclair","orcid":"0000-0002-9936-436X","position":4,"is_corresponding":false},{"id":709289,"name":"Emily M. Venable","orcid":"0000-0002-7436-5817","position":5,"is_corresponding":false},{"id":709290,"name":"Katia S. Chadaideh","orcid":"0000-0003-2251-1170","position":6,"is_corresponding":false},{"id":44563,"name":"Rachel N. Carmody","orcid":"0000-0001-7505-9646","position":7,"is_corresponding":false},{"id":340012,"name":"Francine Grodstein","orcid":"0000-0002-2699-7051","position":8,"is_corresponding":false},{"id":264661,"name":"James R. Mitchell","orcid":"0000-0003-1565-603X","position":9,"is_corresponding":false},{"id":34346,"name":"Yang-Yu Liu","orcid":"0000-0003-2728-4907","position":10,"is_corresponding":false},{"id":580698,"name":"Shanlin Ke","orcid":"0000-0003-4101-0574","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-18T23:46:41.424716Z","pmid":"34579167","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}