{"doi":"10.3390/nu12123697","title":"DNA Methylation Profiles of Vegans and Non-Vegetarians in the Adventist Health Study-2 Cohort","abstract":"We sought to determine if DNA methylation patterns differed between vegans and non-vegetarians in the Adventist Health Study-2 cohort. Genome-wide DNA methylation derived from buffy coat was profiled in 62 vegans and 142 non-vegetarians. Using linear regression, methylation of CpG sites and genes was categorized or summarized according to various genic/intergenic regions and CpG island-related regions, as well as the promoter. Methylation of genes was measured as the average methylation of available CpG’s annotated to the nominated region of the respective gene. A permutation method defining the null distribution adapted from Storey et al. was used to adjust for false discovery. Differences in methylation of several CpG sites and genes were detected at a false discovery rate < 0.05 in region-specific and overall analyses. A vegan diet was associated predominantly with hypomethylation of genes, most notably methyltransferase-like 1 (METTL1). Although a limited number of differentially methylated features were detected in the current study, the false discovery method revealed that a much larger proportion of differentially methylated genes and sites exist, and could be detected with a larger sample size. Our findings suggest modest differences in DNA methylation in vegans and non-vegetarians, with a much greater number of detectable significant differences expected with a larger sample.","journal":"Nutrients","year":2020,"id":68053,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9312,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":270571,"name":"Andrew Mashchak","orcid":"0000-0002-0684-1148","position":1,"is_corresponding":false},{"id":360825,"name":"Valery Filippov","orcid":"0000-0002-7619-9031","position":2,"is_corresponding":false},{"id":270570,"name":"Michael J. Orlich","orcid":"0000-0001-7761-0622","position":3,"is_corresponding":false},{"id":360826,"name":"Penelope J. Duerksen-Hughes","orcid":"0000-0002-0998-2157","position":4,"is_corresponding":false},{"id":360827,"name":"Xin Chen","orcid":"0000-0003-3304-8943","position":5,"is_corresponding":false},{"id":24562,"name":"Charles Wang","orcid":null,"position":6,"is_corresponding":false},{"id":360828,"name":"Kimberly D. Siegmund","orcid":"0000-0001-7599-9726","position":7,"is_corresponding":false},{"id":270568,"name":"Gary E. Fraser","orcid":"0000-0002-5577-3524","position":8,"is_corresponding":false},{"id":360824,"name":"Fayth L. Miles","orcid":"0000-0002-4955-7622","position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":null,"created_at":"2026-07-18T21:40:52.680475Z","pmid":"33266012","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}