{"doi":"10.3390/mps3030061","title":"Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample","abstract":"A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses.","journal":"Methods and Protocols","year":2020,"id":69929,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":369915,"name":"Yurika Otoki","orcid":"0000-0001-9172-2161","position":1,"is_corresponding":false},{"id":369916,"name":"Shannon McClorry","orcid":"0000-0003-0083-9626","position":2,"is_corresponding":false},{"id":369917,"name":"Laurynne C. Coates","orcid":"0000-0002-8325-107X","position":3,"is_corresponding":false},{"id":369918,"name":"Rachel L. Lombardi","orcid":"0000-0001-8917-3810","position":4,"is_corresponding":false},{"id":369919,"name":"Ameer Y. Taha","orcid":"0000-0003-4611-7450","position":5,"is_corresponding":false},{"id":235508,"name":"Carolyn M. Slupsky","orcid":"0000-0002-5100-1594","position":6,"is_corresponding":false},{"id":302303,"name":"Yu Hasegawa","orcid":"0000-0002-2666-162X","position":0,"is_corresponding":true}],"reference_count":9,"raw_metadata":null,"created_at":"2026-07-18T21:42:48.813846Z","pmid":"32859006","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}