{"doi":"10.3390/molecules25245897","title":"Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy","abstract":"<jats:p>Correlative light and electron microscopy (CLEM) is revolutionizing how cell samples are studied. CLEM provides a combination of the molecular and ultrastructural information about a cell. For the execution of CLEM experiments, multimodal fiducial landmarks are applied to precisely overlay light and electron microscopy images. Currently applied fiducials such as quantum dots and organic dye-labeled nanoparticles can be irreversibly quenched by electron beam exposure during electron microscopy. Generally, the sample is therefore investigated with a light microscope first and later with an electron microscope. A versatile fiducial landmark should offer to switch back from electron microscopy to light microscopy while preserving its fluorescent properties. Here, we evaluated green fluorescent and electron dense nanodiamonds for the execution of CLEM experiments and precisely correlated light microscopy and electron microscopy images. We demonstrated that green color emitting fluorescent nanodiamonds withstand electron beam exposure, harsh chemical treatments, heavy metal straining, and, importantly, their fluorescent properties remained intact for light microscopy.</jats:p>","journal":"Molecules","year":2020,"id":25028,"datarank":0.6110318567349704,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.23829585926677024,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.23829585926677024,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":10,"citers_with_citation_signal":10,"citers_with_endowment":10,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":148403,"name":"Markus Peurla","orcid":null,"position":1,"is_corresponding":false},{"id":148404,"name":"Olga Shenderova","orcid":null,"position":2,"is_corresponding":false},{"id":148405,"name":"Jessica M. Rosenholm","orcid":"0000-0001-6085-1112","position":3,"is_corresponding":false},{"id":148402,"name":"Neeraj Prabhakar","orcid":"0000-0002-6470-9858","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33322105","pmcid":"PMC7764487","openalex_id":"https://openalex.org/W3111994488","authors":[],"funders":[{"funder_name":"NordForsk","grant_id":"85352","title":null},{"funder_name":"Academy of Finland","grant_id":"309374","title":"Phenotypic Screening for Cancer Drug Discovery / Consortium: PESCADoR"},{"funder_name":"Sigrid Juséliuksen Säätiö","grant_id":"N/A","title":null},{"funder_name":"National Institutes of Health","grant_id":"3R43CA232901-01S1","title":"Fluorescent Nanodiamonds for Multiplexed Imaging and Diagnostics"}],"total_grants":4,"fwci":0.6516,"citation_percentile":0.6428327,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/1420-3049/25/24/5897/pdf?version=1608118241","host_type":"journal"},{"url":"https://www.mdpi.com/1420-3049/25/24/5897/pdf?version=1608118241","host_type":"GOLD"},{"url":"https://www.mdpi.com/1420-3049/25/24/5897/pdf?version=1608118241","host_type":"publisher"},{"url":"https://www.mdpi.com/1420-3049/25/24/5897/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/molecules25245897","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33322105","host_type":"repository"},{"url":"https://research.abo.fi/en/publications/ff199c41-42dc-4399-a1bd-94d08bae60df","host_type":"repository"},{"url":"https://doaj.org/article/ed0bacddb2dc4b04bdf550e631456ea6","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/molecules25245897","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7764487","host_type":"repository"},{"url":"https://www.mdpi.com/1420-3049/25/24/5897","host_type":"repository"},{"url":"https://www.utupub.fi/handle/10024/157496","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7764487","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7764487?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.3390/molecules25245897","host_type":""},{"url":"http://juuli.fi/Record/0372288620","host_type":""}],"fields_of_study":["Diamond and Carbon-based Materials Research","Force Microscopy Techniques and Applications","Advanced Electron Microscopy Techniques and Applications","Medicine","Materials Science","Physics","Engineering","0301 basic medicine","0303 health sciences","03 medical and health sciences","Cell Line, Tumor","Coloring Agents","Electrons","Humans","Image Processing, Computer-Assisted","Microscopy, Electron","Microscopy, Fluorescence","Nanodiamonds","Single-Cell Analysis"],"mesh_terms":["Coloring Agents","Electrons","Humans","Image Processing, Computer-Assisted","Microscopy, Electron","Microscopy, Fluorescence","Cell Line, Tumor","Nanodiamonds","Single-Cell Analysis"],"keywords":["Electron microscope","Microscopy","Fluorescence microscope","Fiducial marker","Fluorescence","Transmission electron microscopy","Materials science","Scanning electron microscope","Optical microscope","Microscope","Nanotechnology","Optics","Physics","Computer science","Nanodiamonds","Fiducial","Clem","Dual Probes","Single-cell Microscopy","Organic chemistry","Electrons","Article","Microscopy, Electron","QD241-441","Microscopy, Fluorescence","Cell Line, Tumor","Image Processing, Computer-Assisted","Humans","Single-Cell Analysis","Coloring Agents","ta116"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T23:53:58.712655Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}