{"doi":"10.3390/molecules25040980","title":"Effects of Manganese Porphyrins on Cellular Sulfur Metabolism","abstract":"Manganese porphyrins (MnPs), MnTE-2-PyP5+, MnTnHex-2-PyP5+ and MnTnBuOE-2-PyP5+, are superoxide dismutase (SOD) mimetics and form a redox cycle between O2 and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H2O2). We previously found that MnPs oxidize hydrogen sulfide (H2S) to polysulfides (PS; H2Sn, n = 2–6) in buffer. Here, we examine the effects of MnPs for 24 h on H2S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H2S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H2S production and increased intracellular PS. H2S metabolism and PS production were unaffected by cellular O2 (5% versus 21% O2), H2O2 or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H2S production in HEK293 cells and that MnPs decrease mitochondrial H2S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H2S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism.","journal":"Molecules","year":2020,"id":82548,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9673,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":425906,"name":"Yan Gao","orcid":"0000-0001-6905-0143","position":1,"is_corresponding":false},{"id":425907,"name":"Andrea K. Steiger","orcid":"0000-0003-1655-7973","position":2,"is_corresponding":false},{"id":325445,"name":"Michael D. Pluth","orcid":"0000-0003-3604-653X","position":3,"is_corresponding":false},{"id":425908,"name":"Charles R. Tessier","orcid":"0000-0001-8931-6834","position":4,"is_corresponding":false},{"id":397142,"name":"Troy A. Markel","orcid":"0000-0002-6429-2976","position":5,"is_corresponding":false},{"id":427181,"name":"David L. Boone","orcid":null,"position":6,"is_corresponding":false},{"id":271705,"name":"Robert V. Stahelin","orcid":"0000-0001-5443-7863","position":7,"is_corresponding":false},{"id":380443,"name":"Ines Batinic̈‐Haberle","orcid":"0000-0001-8037-1861","position":8,"is_corresponding":false},{"id":427182,"name":"Karl D. Straubg","orcid":null,"position":9,"is_corresponding":false},{"id":425905,"name":"Kenneth R. Olson","orcid":"0000-0003-0178-2203","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-18T21:53:48.608934Z","pmid":"32098303","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}