{"doi":"10.3390/microorganisms9091882","title":"Pochonia chlamydosporia Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens","abstract":"<jats:p>Pochonia chlamydosporia is a fungal parasite of nematode eggs. Studies have shown that some strains of Pochonia chlamydosporia can promote plant growth and induce plants’ systemic resistance to root-knot nematodes by colonizing in their roots. This study aimed to verify the effect of the PC-170 strain on tomato growth and systemic resistance. Split-root experiments were conducted to observe the systemic resistance induced by PC-170. To explore the defense pathway that was excited due to the colonization by PC-170, we tested the expression of marker genes for defense pathways, and used mutant lines to verify the role of plant defense pathways. Our results showed that PC-170 can colonize roots, and promotes growth. We found a role for jasmonic acid (JA) in modulating tomato colonization by PC-170. PC-170 can activate tomato defense responses to reduce susceptibility to infection by the root-knot nematode Meloidogyne incognita, and induced resistance to some pathogens in tomatoes. The marker genes of the defense pathway were significantly induced after PC-170 colonization. However, salicylic acid (SA)- and jasmonic acid (JA)-dependent defenses in roots were variable with the invasion of different pathogens. Defense pathways play different roles at different points in time. SA- and JA-dependent defense pathways were shown to cross-communicate. Different phytohormones have been involved in tomato plants’ responses against different pathogens. Our study confirmed that adaptive JA signaling is necessary to regulate PC-170 colonization and induce systemic resistance in tomatoes.</jats:p>","journal":"Microorganisms","year":2021,"id":606306,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1045292,"name":"Jianlong Zhao","orcid":"0000-0002-8179-6885","position":1,"is_corresponding":false},{"id":1436952,"name":"Miao Bai","orcid":"0000-0003-3786-5326","position":2,"is_corresponding":false},{"id":1556485,"name":"Xing-Xing Ping","orcid":null,"position":3,"is_corresponding":false},{"id":1412491,"name":"Yan-Lin Li","orcid":"0000-0002-1722-4188","position":4,"is_corresponding":false},{"id":1556487,"name":"Yu-Hong Yang","orcid":null,"position":5,"is_corresponding":false},{"id":1556489,"name":"Zhen-Chuan Mao","orcid":null,"position":6,"is_corresponding":false},{"id":1556490,"name":"Guo-Shun Yang","orcid":null,"position":7,"is_corresponding":false},{"id":1556492,"name":"Bing-Yan Xie","orcid":null,"position":8,"is_corresponding":false},{"id":1556482,"name":"Xia Zhuang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Pochonia chlamydosporia Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens","abstract":"<jats:p>Pochonia chlamydosporia is a fungal parasite of nematode eggs. Studies have shown that some strains of Pochonia chlamydosporia can promote plant growth and induce plants’ systemic resistance to root-knot nematodes by colonizing in their roots. This study aimed to verify the effect of the PC-170 strain on tomato growth and systemic resistance. Split-root experiments were conducted to observe the systemic resistance induced by PC-170. To explore the defense pathway that was excited due to the colonization by PC-170, we tested the expression of marker genes for defense pathways, and used mutant lines to verify the role of plant defense pathways. Our results showed that PC-170 can colonize roots, and promotes growth. We found a role for jasmonic acid (JA) in modulating tomato colonization by PC-170. PC-170 can activate tomato defense responses to reduce susceptibility to infection by the root-knot nematode Meloidogyne incognita, and induced resistance to some pathogens in tomatoes. The marker genes of the defense pathway were significantly induced after PC-170 colonization. However, salicylic acid (SA)- and jasmonic acid (JA)-dependent defenses in roots were variable with the invasion of different pathogens. Defense pathways play different roles at different points in time. SA- and JA-dependent defense pathways were shown to cross-communicate. Different phytohormones have been involved in tomato plants’ responses against different pathogens. Our study confirmed that adaptive JA signaling is necessary to regulate PC-170 colonization and induce systemic resistance in tomatoes.</jats:p>","is_dataset_classified":null,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34576777","pmcid":"PMC8470021","openalex_id":"https://openalex.org/W3198321651","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"32072396","title":null},{"funder_name":"the National Key R &amp; D Program","grant_id":"2017YFD0200600","title":null},{"funder_name":"the National Key R &amp; D Program","grant_id":"2016YFD0201000","title":null},{"funder_name":"Agriculture Research System of China","grant_id":"CARS-25","title":null}],"total_grants":4,"fwci":1.8437,"citation_percentile":0.860158,"influential_citations":0,"citation_trend":[{"year":2022,"count":2},{"year":2023,"count":2},{"year":2024,"count":5},{"year":2025,"count":7}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2076-2607/9/9/1882/pdf","host_type":"journal"},{"url":"https://www.mdpi.com/2076-2607/9/9/1882/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/microorganisms9091882","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34576777","host_type":"repository"},{"url":"https://doaj.org/article/fb0ab0806a1c4bca860fdc44bb743bd3","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC8470021","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/microorganisms9091882","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8470021","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8470021","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8470021?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Nematode management and characterization studies","Legume Nitrogen Fixing Symbiosis","Plant-Microbe Interactions and Immunity"],"mesh_terms":[],"keywords":["Jasmonic acid","Biology","Meloidogyne incognita","Plant defense against herbivory","Root-knot nematode","Defence mechanisms","Salicylic acid","Colonization","Nematode","Gene","Systemic acquired resistance","Nematode infection","Lycopersicon","Microbiology","Botany","Mutant","Genetics","Arabidopsis","Salicylic acid (SA)","Pathogen","Induced Defenses","Pochonia Chlamydosporia","Jasmonic Acid (Ja)","Root Endophytes"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T04:16:49.883801Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}