{"doi":"10.3390/microorganisms8020295","title":"Proteomic and Transcriptional Profiles of Human Stem Cell-Derived β Cells Following Enteroviral Challenge","abstract":"Enteroviral infections are implicated in islet autoimmunity and type 1 diabetes (T1D) pathogenesis. Significant β-cell stress and damage occur with viral infection, leading to cells that are dysfunctional and vulnerable to destruction. Human stem cell-derived β (SC-β) cells are insulin-producing cell clusters that closely resemble native β cells. To better understand the events precipitated by enteroviral infection of β cells, we investigated transcriptional and proteomic changes in SC-β cells challenged with coxsackie B virus (CVB). We confirmed infection by demonstrating that viral protein colocalized with insulin-positive SC-β cells by immunostaining. Transcriptome analysis showed a decrease in insulin gene expression following infection, and combined transcriptional and proteomic analysis revealed activation of innate immune pathways, including type I interferon (IFN), IFN-stimulated genes, nuclear factor-kappa B (NF-κB) and downstream inflammatory cytokines, and major histocompatibility complex (MHC) class I. Finally, insulin release by CVB4-infected SC-β cells was impaired. These transcriptional, proteomic, and functional findings are in agreement with responses in primary human islets infected with CVB ex vivo. Human SC-β cells may serve as a surrogate for primary human islets in virus-induced diabetes models. Because human SC-β cells are more genetically tractable and accessible than primary islets, they may provide a preferred platform for investigating T1D pathogenesis and developing new treatments.","journal":"Microorganisms","year":2020,"id":86144,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":440793,"name":"Agata Jurczyk","orcid":null,"position":1,"is_corresponding":false},{"id":440794,"name":"Basanthi Satish","orcid":null,"position":2,"is_corresponding":false},{"id":439665,"name":"Sambra D. Redick","orcid":"0000-0003-1462-2022","position":3,"is_corresponding":false},{"id":439666,"name":"Natasha Qaisar","orcid":"0000-0001-6838-941X","position":4,"is_corresponding":false},{"id":440795,"name":"Melanie I. Trombly","orcid":null,"position":5,"is_corresponding":false},{"id":284600,"name":"Pranitha Vangala","orcid":"0000-0003-4000-7891","position":6,"is_corresponding":false},{"id":440796,"name":"Riccardo Racicot","orcid":null,"position":7,"is_corresponding":false},{"id":440797,"name":"Rita Bortell","orcid":null,"position":8,"is_corresponding":false},{"id":295051,"name":"David M. Harlan","orcid":"0000-0002-6938-8109","position":9,"is_corresponding":false},{"id":282172,"name":"Dale L. Greiner","orcid":"0000-0002-5923-339X","position":10,"is_corresponding":false},{"id":263551,"name":"Michael A. Brehm","orcid":"0000-0001-6813-3262","position":11,"is_corresponding":false},{"id":439667,"name":"Jerry L. Nadler","orcid":"0000-0002-4200-6991","position":12,"is_corresponding":false},{"id":104332,"name":"Jennifer Wang","orcid":"0000-0002-2556-9100","position":13,"is_corresponding":false},{"id":439664,"name":"Julius O. Nyalwidhe","orcid":"0000-0003-2854-7510","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-18T21:58:41.997408Z","pmid":"32093375","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}