{"doi":"10.3390/microorganisms13050976","title":"On-Site Dual Detection of Airborne Acinetobacter baumannii and Its Carbapenem-Resistant Gene blaOXA-23 Using a One-Pot Visual LAMP-CRISPR/Cas12a-Based Platform","abstract":"<jats:p>Acinetobacter baumannii (A. baumannii), a very common pathogen, poses a significant public health threat due to its antibiotic resistance and long survival in healthcare environments. Both A. baumannii and carbapenem-resistant A. baumannii (CRAB) can spread through the air, increasing infection risks. Therefore, monitoring their presence in the air is of great significance, especially in hospitals. Herein, we developed a Chelex-100-LAMP-CRISPR/Cas12a (CLC) platform including DNA release and nucleic acid test. Combined with a wet cyclone sampler, the platform can detect airborne A. baumannii and its most common carbapenem-resistant gene, blaOXA-23, within 70 min. This CLC platform has also been proven to have a detection limit of 6 × 102 CFU of CRAB per test through simulated air samples. Moreover, this platform was also used to test five actual air samples from a tertiary hospital, and the results achieved perfect concordance with sequencing data, validating the platform’s accuracy and reliability. Therefore, the CLC platform showed great potential for the rapid, on-site detection of airborne A. baumannii and its carbapenem-resistant gene blaOXA-23, offering a valuable tool for infection control in healthcare environments.</jats:p>","journal":"Microorganisms","year":2025,"id":662661,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":486367,"name":"Tong Zhang","orcid":"0000-0002-5511-2558","position":1,"is_corresponding":false},{"id":1475567,"name":"Wei Huang","orcid":"0000-0003-2555-0140","position":2,"is_corresponding":false},{"id":1729988,"name":"Jinhui Zhu","orcid":null,"position":3,"is_corresponding":false},{"id":1729990,"name":"Haoran Qin","orcid":null,"position":4,"is_corresponding":false},{"id":584609,"name":"Xi Chen","orcid":"0000-0002-6378-4557","position":5,"is_corresponding":false},{"id":132484,"name":"Wang Zhao","orcid":"0000-0003-0848-5738","position":6,"is_corresponding":false},{"id":1729992,"name":"Guodong Sui","orcid":null,"position":7,"is_corresponding":false},{"id":1542980,"name":"Huijun Lu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"On-Site Dual Detection of Airborne Acinetobacter baumannii and Its Carbapenem-Resistant Gene blaOXA-23 Using a One-Pot Visual LAMP-CRISPR/Cas12a-Based Platform","abstract":"<jats:p>Acinetobacter baumannii (A. baumannii), a very common pathogen, poses a significant public health threat due to its antibiotic resistance and long survival in healthcare environments. Both A. baumannii and carbapenem-resistant A. baumannii (CRAB) can spread through the air, increasing infection risks. Therefore, monitoring their presence in the air is of great significance, especially in hospitals. Herein, we developed a Chelex-100-LAMP-CRISPR/Cas12a (CLC) platform including DNA release and nucleic acid test. Combined with a wet cyclone sampler, the platform can detect airborne A. baumannii and its most common carbapenem-resistant gene, blaOXA-23, within 70 min. This CLC platform has also been proven to have a detection limit of 6 × 102 CFU of CRAB per test through simulated air samples. Moreover, this platform was also used to test five actual air samples from a tertiary hospital, and the results achieved perfect concordance with sequencing data, validating the platform’s accuracy and reliability. Therefore, the CLC platform showed great potential for the rapid, on-site detection of airborne A. baumannii and its carbapenem-resistant gene blaOXA-23, offering a valuable tool for infection control in healthcare environments.</jats:p>","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40431150","pmcid":"PMC12114085","openalex_id":"https://openalex.org/W4409766728","authors":[],"funders":[{"funder_name":"Shanghai Municipal Science and Technology Major Project","grant_id":"ZD2021CY001","title":null},{"funder_name":"Shanghai Municipal Science and Technology Major Project","grant_id":"GZNL2024A01007","title":null},{"funder_name":"Shanghai Sci-Tech Inno Center for Infection & Immunity","grant_id":"SSIII-202407","title":null}],"total_grants":3,"fwci":1.5785,"citation_percentile":0.81220883,"influential_citations":0,"citation_trend":[{"year":2026,"count":5}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2076-2607/13/5/976/pdf?version=1745497933","host_type":"journal"},{"url":"https://www.mdpi.com/2076-2607/13/5/976/pdf?version=1745497933","host_type":"publisher"},{"url":"https://www.mdpi.com/2076-2607/13/5/976/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/microorganisms13050976","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/40431150","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12114085","host_type":"repository"},{"url":"https://doaj.org/article/a448f1a1a66e4f539b3215522b4f1348","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC12114085","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12114085?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Biosensors and Analytical Detection","Antibiotic Resistance in Bacteria","Vibrio bacteria research studies"],"mesh_terms":[],"keywords":["Acinetobacter baumannii","Microbiology","Acinetobacter","CRISPR","Biology","Gene","Bacteria","Pseudomonas aeruginosa","Genetics","Antibiotics","Nucleic Acid Detection","Airborne Pathogen","Carbapenem-resistant Gene","The Clc Platform"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T16:48:17.734354Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}