{"doi":"10.3390/mi11070663","title":"Three-Dimensional Regeneration of Patient-Derived Intestinal Organoid Epithelium in a Physiodynamic Mucosal Interface-on-a-Chip","abstract":"The regeneration of the mucosal interface of the human intestine is critical in the host-gut microbiome crosstalk associated with gastrointestinal diseases. The biopsy-derived intestinal organoids provide genetic information of patients with physiological cytodifferentiation. However, the enclosed lumen and static culture condition substantially limit the utility of patient-derived organoids for microbiome-associated disease modeling. Here, we report a patient-specific three-dimensional (3D) physiodynamic mucosal interface-on-a-chip (PMI Chip) that provides a microphysiological intestinal milieu under defined biomechanics. The real-time imaging and computational simulation of the PMI Chip verified the recapitulation of non-linear luminal and microvascular flow that simulates the hydrodynamics in a living human gut. The multiaxial deformations in a convoluted microchannel not only induced dynamic cell strains but also enhanced particle mixing in the lumen microchannel. Under this physiodynamic condition, an organoid-derived epithelium obtained from the patients diagnosed with Crohn's disease, ulcerative colitis, or colorectal cancer independently formed 3D epithelial layers with disease-specific differentiations. Moreover, co-culture with the human fecal microbiome in an anoxic-oxic interface resulted in the formation of stochastic microcolonies without a loss of epithelial barrier function. We envision that the patient-specific PMI Chip that conveys genetic, epigenetic, and environmental factors of individual patients will potentially demonstrate the pathophysiological dynamics and complex host-microbiome crosstalk to target a patient-specific disease modeling.","journal":"Micromachines","year":2020,"id":56076,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":88,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9488,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":291186,"name":"Woojung Shin","orcid":"0000-0002-1780-0317","position":1,"is_corresponding":false},{"id":291187,"name":"Domin Koh","orcid":"0000-0002-6515-5543","position":2,"is_corresponding":false},{"id":291188,"name":"Alexander Wu","orcid":"0000-0001-5106-132X","position":3,"is_corresponding":false},{"id":291189,"name":"Yoko M. Ambrosini","orcid":"0000-0002-9543-2660","position":4,"is_corresponding":false},{"id":291190,"name":"So‐Youn Min","orcid":"0000-0002-5436-6089","position":5,"is_corresponding":false},{"id":291191,"name":"Sabine Eckhardt","orcid":"0000-0001-6958-5375","position":6,"is_corresponding":false},{"id":291192,"name":"Rachel Fleming","orcid":"0000-0003-1038-053X","position":7,"is_corresponding":false},{"id":291193,"name":"Seung Kim","orcid":"0000-0003-4373-9828","position":8,"is_corresponding":false},{"id":291194,"name":"Sowon Park","orcid":"0000-0002-2498-8004","position":9,"is_corresponding":false},{"id":291195,"name":"Hong Koh","orcid":"0000-0002-3660-7483","position":10,"is_corresponding":false},{"id":291196,"name":"Tae Kyung Yoo","orcid":"0000-0003-4394-9454","position":11,"is_corresponding":false},{"id":291197,"name":"Hyun Jung Kim","orcid":"0000-0001-5342-2870","position":12,"is_corresponding":false},{"id":291185,"name":"Yong Cheol Shin","orcid":"0000-0002-1418-5929","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T21:05:49.581291Z","pmid":"32645991","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}