{"doi":"10.3390/metabo12090862","title":"Sugarcane Metabolome Compositional Stability in Pretreatment Processes for NMR Measurements","abstract":"<jats:p>Sugarcane is essential for global sugar production and its compressed juice is a key raw material for industrial products. Sugarcane juice includes various metabolites with abundances and compositional balances influencing product qualities and functionalities. Therefore, understanding the characteristic features of the sugarcane metabolome is important. However, sugarcane compositional variability and stability, even in pretreatment processes for nuclear magnetic resonance (NMR)-based metabolomic studies, remains elusive. The objective of this study is to evaluate sugarcane juice metabolomic variability affected by centrifugation, filtration, and thermal pretreatments, as well as the time-course changes for determining optimal conditions for NMR-based metabolomic approach. The pretreatment processes left the metabolomic compositions unchanged, indicating that these pretreatments are compatible with one another and the studied metabolomes are comparable. The thermal processing provided stability to the metabolome for more than 32 h at room temperature. Based on the determined analytical conditions, we conducted an NMR-based metabolomic study to discriminate the differences in the harvest period and allowed for successfully identifying the characteristic metabolome. Our findings denote that NMR-based sugarcane metabolomics enable us to provide an opportunity to collect a massive amount of data upon collaboration between multiple researchers, resulting in the rapid construction of useful databases for both research purposes and industrial use.</jats:p>","journal":"Metabolites","year":2022,"id":14188,"datarank":0.432655449811047,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.12073921855907158,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.12073921855907158,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":5,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":113227,"name":"Chiaki Ishikawa","orcid":"0009-0001-2960-6909","position":1,"is_corresponding":false},{"id":113228,"name":"Makoto Umeda","orcid":null,"position":2,"is_corresponding":false},{"id":113229,"name":"Yusuke Tarumoto","orcid":null,"position":3,"is_corresponding":false},{"id":113230,"name":"Megumi Okubo","orcid":null,"position":4,"is_corresponding":false},{"id":113231,"name":"Yasuaki Tamura","orcid":null,"position":5,"is_corresponding":false},{"id":113232,"name":"Hiroshi Ono","orcid":null,"position":6,"is_corresponding":false},{"id":113226,"name":"Yasuhiro Date","orcid":"0000-0001-9645-4370","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36144266","pmcid":"PMC9503584","openalex_id":"https://openalex.org/W4295779760","authors":[],"funders":[{"funder_name":"Japan Society for the Promotion of Science","grant_id":"KAKENHI JP21K05553","title":null},{"funder_name":"Japan Society for the Promotion of Science","grant_id":"JPJ008617. 18063550","title":null}],"total_grants":2,"fwci":1.4698,"citation_percentile":0.83782269,"influential_citations":1,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":4},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2218-1989/12/9/862/pdf?version=1663162013","host_type":"journal"},{"url":"https://www.mdpi.com/2218-1989/12/9/862/pdf?version=1663162013","host_type":"GOLD"},{"url":"https://www.mdpi.com/2218-1989/12/9/862/pdf?version=1663162013","host_type":"publisher"},{"url":"https://www.mdpi.com/2218-1989/12/9/862/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/metabo12090862","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36144266","host_type":"repository"},{"url":"https://doaj.org/article/995b65fe2941422791a3bf8f8aa3cba8","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/metabo12090862","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9503584","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9503584","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9503584?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Sugarcane Cultivation and Processing","Natural Products and Biological Research","Spectroscopy and Chemometric Analyses","Medicine","Chemistry","Environmental Science"],"mesh_terms":[],"keywords":["Metabolome","Metabolomics","Chemistry","Metabolite","Nuclear magnetic resonance spectroscopy","Biochemical engineering","Food science","Chromatography","Biochemistry","Organic chemistry","Nuclear magnetic resonance","Harvest period","Support vector machine","Machine Learning","Sugarcane Juice","Metabolite Composition"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Reduced inequalities"},{"sdg_number":0,"sdg_label":"Peace, Justice and strong institutions"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T05:48:27.141115Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}