{"doi":"10.3390/jcm9041221","title":"Local Serpin Treatment via Chitosan-Collagen Hydrogel after Spinal Cord Injury Reduces Tissue Damage and Improves Neurologic Function","abstract":"Spinal cord injury (SCI) results in massive secondary damage characterized by a prolonged inflammation with phagocytic macrophage invasion and tissue destruction. In prior work, sustained subdural infusion of anti-inflammatory compounds reduced neurological deficits and reduced pro-inflammatory cell invasion at the site of injury leading to improved outcomes. We hypothesized that implantation of a hydrogel loaded with an immune modulating biologic drug, Serp-1, for sustained delivery after crush-induced SCI would have an effective anti-inflammatory and neuroprotective effect. Rats with dorsal column SCI crush injury, implanted with physical chitosan-collagen hydrogels (CCH) had severe granulomatous infiltration at the site of the dorsal column injury, which accumulated excess edema at 28 days post-surgery. More pronounced neuroprotective changes were observed with high dose (100 µg/50 µL) Serp-1 CCH implanted rats, but not with low dose (10 µg/50 µL) Serp-1 CCH. Rats treated with Serp-1 CCH implants also had improved motor function up to 20 days with recovery of neurological deficits attributed to inhibition of inflammation-associated tissue damage. In contrast, prolonged low dose Serp-1 infusion with chitosan did not improve recovery. Intralesional implantation of hydrogel for sustained delivery of the Serp-1 immune modulating biologic offers a neuroprotective treatment of acute SCI.","journal":"Journal of Clinical Medicine","year":2020,"id":98430,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9582,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":485394,"name":"Liqiang Zhang","orcid":"0000-0002-4175-7590","position":1,"is_corresponding":false},{"id":392469,"name":"Jordan R. Yaron","orcid":"0000-0002-4133-474X","position":2,"is_corresponding":false},{"id":393958,"name":"Lauren N. Schutz","orcid":null,"position":3,"is_corresponding":false},{"id":485976,"name":"Christian J. Kwiecien-Delaney","orcid":null,"position":4,"is_corresponding":false},{"id":393959,"name":"Enkidia A. Awo","orcid":null,"position":5,"is_corresponding":false},{"id":392471,"name":"Michelle Burgin","orcid":"0000-0002-8596-9683","position":6,"is_corresponding":false},{"id":485395,"name":"Wojciech Dąbrowski","orcid":"0000-0003-0449-2375","position":7,"is_corresponding":false},{"id":392477,"name":"Alexandra Lucas","orcid":"0000-0003-3869-5373","position":8,"is_corresponding":false},{"id":392474,"name":"Jacek M. Kwiecień","orcid":"0000-0002-0128-9307","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-18T22:36:34.157932Z","pmid":"32340262","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}