{"doi":"10.3390/jcm11247285","title":"A PPARG Splice Variant in Granulosa Cells Is Associated with Polycystic Ovary Syndrome","abstract":"<jats:p>Background: We explored whether there are splice variants (SVs) of peroxisome proliferator-activated receptor-gamma (PPARG) in polycystic ovary syndrome (PCOS) patients and its relationship with clinical features and KGN cell functions. Methods: We performed a study involving 153 women with PCOS and 153 age-matched controls. One type of PPARG SV was detected by SMARTer RACE. The correlations between PPARG SV expression levels, clinical features, and KGN cell functions were analyzed. The effect of the PPARG SV on the expression of important genes in metabolism-related pathways was explored by PCR array. Results: The expression of the PPARG SV in PCOS patients was significantly higher than that in the controls. Clinical features were more significant in the PCOS group with the SV. Compared with overexpression of PPARG, the overexpression of the PPARG SV inhibited the proliferation, migration, and apoptosis of KGN cells in vitro. The genes related to the PPARG SV were mainly involved in lipid metabolism. Conclusion: While granulosa cells contribute greatly to the development of follicles, our results suggest that the identified PPARG SV may regulate cell proliferation, migration, and apoptosis in granulosa cells, which could partially explain the mechanisms of ovulation dysfunction in PCOS. Further investigation of the utility of this PPARG SV as a biomarker for PCOS is warranted.</jats:p>","journal":"Journal of Clinical Medicine","year":2022,"id":638495,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1658386,"name":"Jing-Jing Xu","orcid":"0000-0002-4496-0228","position":1,"is_corresponding":false},{"id":457177,"name":"Cheng Li","orcid":"0000-0002-1286-9403","position":2,"is_corresponding":false},{"id":1658387,"name":"Jia-Le Yu","orcid":null,"position":3,"is_corresponding":false},{"id":1658389,"name":"Yan-Ting Wu","orcid":"0000-0002-2293-1792","position":4,"is_corresponding":false},{"id":1658391,"name":"He-Feng Huang","orcid":null,"position":5,"is_corresponding":false},{"id":1658385,"name":"Chao-Yi Shi","orcid":"0000-0002-5967-6377","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A PPARG Splice Variant in Granulosa Cells Is Associated with Polycystic Ovary Syndrome","abstract":"<jats:p>Background: We explored whether there are splice variants (SVs) of peroxisome proliferator-activated receptor-gamma (PPARG) in polycystic ovary syndrome (PCOS) patients and its relationship with clinical features and KGN cell functions. Methods: We performed a study involving 153 women with PCOS and 153 age-matched controls. One type of PPARG SV was detected by SMARTer RACE. The correlations between PPARG SV expression levels, clinical features, and KGN cell functions were analyzed. The effect of the PPARG SV on the expression of important genes in metabolism-related pathways was explored by PCR array. Results: The expression of the PPARG SV in PCOS patients was significantly higher than that in the controls. Clinical features were more significant in the PCOS group with the SV. Compared with overexpression of PPARG, the overexpression of the PPARG SV inhibited the proliferation, migration, and apoptosis of KGN cells in vitro. The genes related to the PPARG SV were mainly involved in lipid metabolism. Conclusion: While granulosa cells contribute greatly to the development of follicles, our results suggest that the identified PPARG SV may regulate cell proliferation, migration, and apoptosis in granulosa cells, which could partially explain the mechanisms of ovulation dysfunction in PCOS. Further investigation of the utility of this PPARG SV as a biomarker for PCOS is warranted.</jats:p>","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36555903","pmcid":"PMC9786670","openalex_id":"https://openalex.org/W4311974439","authors":[],"funders":[{"funder_name":"National Key Research and Development Program of China","grant_id":"2021YFC2700701","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"81661128010","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"82171686","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"82001571","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"82088102","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"81671412","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"18410711800","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"20XD1424100","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"SHDC12018X17","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"SHDC12019107","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2019-I2M-5-064","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2020CXJQ01","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"SHDC2020CR1008A","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"JQ201717","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"PPXK2018-06","title":null},{"funder_name":"Clinical Research Plan of Shanghai Shenkang Hospital Development Center","grant_id":"SHDC12018X17, SHDC12019107","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"81661128010, 82171686, 82001571, 82088102, 81671412","title":null}],"total_grants":17,"fwci":0.5487,"citation_percentile":0.61577022,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":4},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/2077-0383/11/24/7285/pdf?version=1670569803","host_type":"journal"},{"url":"https://www.mdpi.com/2077-0383/11/24/7285/pdf?version=1670569803","host_type":"publisher"},{"url":"https://www.mdpi.com/2077-0383/11/24/7285/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/jcm11247285","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36555903","host_type":"repository"},{"url":"https://doaj.org/article/e249b1f265e648879b5b41be8bbea19f","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/jcm11247285","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9786670","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9786670","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9786670?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Peroxisome Proliferator-Activated Receptors","Ovarian function and disorders","Lipid metabolism and biosynthesis"],"mesh_terms":[],"keywords":["Peroxisome proliferator-activated receptor gamma","Polycystic ovary","Internal medicine","Medicine","Endocrinology","Peroxisome proliferator-activated receptor","Ovary","Biomarker","Receptor","Biology","Insulin resistance","Genetics","Obesity","Apoptosis","Migration","Proliferation","Splice variant","PCOS","lipid metabolism","Pparg"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"},{"name":"uniprot"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T20:46:28.109703Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}