{"doi":"10.3390/ijms252313038","title":"Neutrophil Elastase Targets Select Proteins on Human Blood-Monocyte-Derived Macrophage Cell Surfaces","abstract":"Neutrophil elastase (NE) has been reported to be a pro-inflammatory stimulus for macrophages. The aim of the present study was to determine the impact of NE exposure on the human macrophage proteome and evaluate its impact on pro-inflammatory signals. Human blood monocytes from healthy volunteers were differentiated to macrophages and then exposed to either 500 nM of NE or control vehicle for 2 h in triplicate. Label-free quantitative proteomics analysis identified 41 differentially expressed proteins in the NE versus control vehicle datasets. A total of 26 proteins were downregulated and of those, 21 were cell surface proteins. Importantly, four of the cell surface proteins were proteoglycans: neuropilin 1 (NRP1), syndecan 2 (SDC2), glypican 4 (GPC4), and CD99 antigen-like protein 2 (CD99L2) along with neuropilin 2 (NRP2), CD99 antigen (CD99), and endoglin (ENG) which are known interactors. Additional NE-targeted proteins related to macrophage function were also measured including CD40, CD48, SPINT1, ST14, and MSR1. Collectively, this study provides a comprehensive unbiased view of selective NE-targeted cell surface proteins in chronically inflamed lungs.","journal":"International Journal of Molecular Sciences","year":2024,"id":466832,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9622,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":332705,"name":"Apparao B. Kummarapurugu","orcid":"0000-0002-6175-5293","position":1,"is_corresponding":false},{"id":426715,"name":"Shuo Zheng","orcid":"0009-0005-7347-3188","position":2,"is_corresponding":false},{"id":452519,"name":"Gamze B. Bulut","orcid":"0000-0002-8344-3338","position":3,"is_corresponding":false},{"id":1298948,"name":"Le Kang","orcid":"0000-0002-0367-2692","position":4,"is_corresponding":false},{"id":1298949,"name":"Aashish Batheja","orcid":"0009-0003-4437-0249","position":5,"is_corresponding":false},{"id":696758,"name":"Adam M. Hawkridge","orcid":"0000-0002-2301-4611","position":6,"is_corresponding":false},{"id":442433,"name":"Judith A. Voynow","orcid":"0000-0002-2437-2859","position":7,"is_corresponding":false},{"id":1298947,"name":"Nadia Tasnim Ahmed","orcid":"0009-0004-9146-9898","position":0,"is_corresponding":true}],"reference_count":65,"raw_metadata":null,"created_at":"2026-07-19T02:05:06.743102Z","pmid":"39684750","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}