{"doi":"10.3390/ijms251910378","title":"Amino Acid-Based Protein-Mimic Hydrogel Incorporating Pro-Regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds","abstract":"Pro-regenerative lipid mediator 1 (PreM1) is a specialized pro-resolving lipid mediator that promotes wound healing and regenerative functions of mesenchymal stem cells (MSCs), endothelial cells, and macrophages. The healing of third-degree (3°) burns and regenerative functions of MSCs are enhanced by ACgel1, an arginine-and-chitosan-based protein-mimic hybrid hydrogel. Adipose-tissue derived microvascular fragments (MVFs) are native vascularization units and a rich source of MSCs, endothelial cells, and perivascular cells for tissue regeneration. Here we describe an innovative PreM1-MVFs-ACgel1 construct that incorporated PreM1 and MVFs into ACgel1 via optimal design and fabrication. This construct delivered PreM1 to 3°-burn wounds at least up to 7 days-post-burn (dpb), and scaffolded and delivered MVFs. PreM1-MVFs-ACgel1 promoted the healing of 3°-burns in mice, including vascularization and collagen formation. The re-epithelization and closure of 3° burn wounds were promoted by ACgel1, MVFs, PreM1, MVFs-ACgel1, PreM1-ACgel1, or PreM1-MVFs-ACgel1 at certain time-point(s), while PreM1-MVFs-ACgel1 was most effective with 97% closure and 4.69% relative epithelial gap at 13 dpb compared to saline control. The PreM1-ACgel1 and MVFs-ACgel1 also promoted blood vessel regeneration of 3°-burns although PreM1-MVFs-ACgel1 is significantly more effective. These PreM1- and/or MVF-functionalized ACgel1 have nonexistent or minimal graft-donor requirements and are promising adjuvant therapeutic candidates for treating deep burns.","journal":"Revista de psicología general y aplicada: Revista de la Federación Española de Asociaciones de Psicología","year":2024,"id":481574,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9623,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1321581,"name":"Shanchun Su","orcid":"0000-0001-7723-5156","position":1,"is_corresponding":false},{"id":1321989,"name":"Chih-Chang Chu","orcid":null,"position":2,"is_corresponding":false},{"id":436644,"name":"Yuichi Kobayashi","orcid":"0000-0002-4385-9531","position":3,"is_corresponding":false},{"id":980852,"name":"Abdul‐Razak Masoud","orcid":"0000-0002-8621-4063","position":4,"is_corresponding":false},{"id":708113,"name":"Hongying Peng","orcid":null,"position":5,"is_corresponding":false},{"id":1321990,"name":"Nathan R. Lien","orcid":null,"position":6,"is_corresponding":false},{"id":1018753,"name":"Mingyu He","orcid":"0000-0001-5108-5145","position":7,"is_corresponding":false},{"id":708116,"name":"Christopher Vuong","orcid":null,"position":8,"is_corresponding":false},{"id":813752,"name":"Ryan Tran","orcid":null,"position":9,"is_corresponding":false},{"id":436642,"name":"Song Hong","orcid":"0000-0002-3801-4147","position":10,"is_corresponding":false},{"id":707388,"name":"Yan Lü","orcid":"0000-0002-2868-2352","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T02:07:10.067421Z","pmid":"39408708","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}