{"doi":"10.3390/ijms25137378","title":"Photophysical Characterization and In Vitro Evaluation of α-Mangostin-Loaded HDL Mimetic Nano-Complex in LN-229 Glioblastoma Spheroid Model","abstract":"Cytotoxic activity has been reported for the xanthone α-mangostin (AMN) against Glioblastoma multiforme (GBM), an aggressive malignant brain cancer with a poor prognosis. Recognizing that AMN's high degree of hydrophobicity is likely to limit its systemic administration, we formulated AMN using reconstituted high-density lipoprotein (rHDL) nanoparticles. The photophysical characteristics of the formulation, including fluorescence lifetime and steady-state anisotropy, indicated that AMN was successfully incorporated into the rHDL nanoparticles. To our knowledge, this is the first report on the fluorescent characteristics of AMN with an HDL-based drug carrier. Cytotoxicity studies in a 2D culture and 3D spheroid model of LN-229 GBM cells and normal human astrocytes showed an enhanced therapeutic index with the rHDL-AMN formulation compared to the unincorporated AMN and Temozolomide, a standard GBM chemotherapy agent. Furthermore, treatment with the rHDL-AMN facilitated a dose-dependent upregulation of autophagy and reactive oxygen species generation to a greater extent in LN-229 cells compared to astrocytes, indicating the reduced off-target toxicity of this novel formulation. These studies indicate the potential therapeutic benefits to GBM patients via selective targeting using the rHDL-AMN formulation.","journal":"International Journal of Molecular Sciences","year":2024,"id":438662,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9558,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":434497,"name":"Nirupama Sabnis","orcid":"0000-0002-8686-1578","position":1,"is_corresponding":false},{"id":1073800,"name":"Akpedje Serena Dossou","orcid":"0000-0002-9844-8860","position":2,"is_corresponding":false},{"id":374085,"name":"Jose Chavez","orcid":"0000-0002-3834-3573","position":3,"is_corresponding":false},{"id":374087,"name":"Luca Ceresa","orcid":"0000-0001-8771-496X","position":4,"is_corresponding":false},{"id":374090,"name":"Zygmunt Gryczyński","orcid":"0000-0003-3801-4582","position":5,"is_corresponding":false},{"id":374088,"name":"Rafał Fudala","orcid":"0000-0001-9558-1394","position":6,"is_corresponding":false},{"id":1249323,"name":"Rob D. Dickerman","orcid":null,"position":7,"is_corresponding":false},{"id":282160,"name":"Bruce A. Bunnell","orcid":"0000-0001-6196-3722","position":8,"is_corresponding":false},{"id":434499,"name":"Andras G. Lacko","orcid":"0000-0001-9168-7802","position":9,"is_corresponding":false},{"id":1073801,"name":"Ammar Kapic","orcid":"0000-0001-9795-1109","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-19T02:00:43.618468Z","pmid":"39000485","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}