{"doi":"10.3390/ijms25116161","title":"Anti-Inflammatory Activity of No-Ozone Cold Plasma in Porphyromonas gingivalis Lipopolysaccharide-Induced Periodontitis Rats","abstract":"<jats:p>Periodontitis is an inflammatory disease caused by Porphyromonas gingivalis (P. gingivalis) in the oral cavity. This periodontal disease causes damage to the periodontal ligament and alveolar bone and can cause tooth loss, but there is no definite treatment yet. In this study, we investigated the possibility of using no-ozone cold plasma to safely treat periodontitis in the oral cavity. First, human gingival fibroblasts (HGFs) were treated with P. gingivalis-derived lipopolysaccharide (PG-LPS) to induce an inflammatory response, and then the anti-inflammatory effect of NCP was examined, and a study was conducted to identify the mechanism of action. Additionally, the anti-inflammatory effect of NCP was verified in rats that developed an inflammatory response similar to periodontitis. When NCP was applied to PG-LPS-treated HGFs, the activities of inflammatory proteins and cytokines were effectively inhibited. It was confirmed that the process of denaturing the medium by charged particles of NCP is essential for the anti-inflammatory effect of NCP. Also, it was confirmed that repeated treatment of periodontitis rats with NCP effectively reduced the inflammatory cells and osteoclast activity. As a result, this study suggests that NCP can be directly helpful in the treatment of periodontitis in the future.</jats:p>","journal":"International Journal of Molecular Sciences","year":2024,"id":634546,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1645783,"name":"Yoon-Seo Jang","orcid":null,"position":1,"is_corresponding":false},{"id":1645784,"name":"Ji-Young Joo","orcid":null,"position":2,"is_corresponding":false},{"id":1645785,"name":"Gyoo-Cheon Kim","orcid":null,"position":3,"is_corresponding":false},{"id":1645786,"name":"Jeong-Hae Choi","orcid":null,"position":4,"is_corresponding":false},{"id":1645782,"name":"Kwang-Ha Park","orcid":"0000-0003-3702-1799","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Anti-Inflammatory Activity of No-Ozone Cold Plasma in Porphyromonas gingivalis Lipopolysaccharide-Induced Periodontitis Rats","abstract":"<jats:p>Periodontitis is an inflammatory disease caused by Porphyromonas gingivalis (P. gingivalis) in the oral cavity. This periodontal disease causes damage to the periodontal ligament and alveolar bone and can cause tooth loss, but there is no definite treatment yet. In this study, we investigated the possibility of using no-ozone cold plasma to safely treat periodontitis in the oral cavity. First, human gingival fibroblasts (HGFs) were treated with P. gingivalis-derived lipopolysaccharide (PG-LPS) to induce an inflammatory response, and then the anti-inflammatory effect of NCP was examined, and a study was conducted to identify the mechanism of action. Additionally, the anti-inflammatory effect of NCP was verified in rats that developed an inflammatory response similar to periodontitis. When NCP was applied to PG-LPS-treated HGFs, the activities of inflammatory proteins and cytokines were effectively inhibited. It was confirmed that the process of denaturing the medium by charged particles of NCP is essential for the anti-inflammatory effect of NCP. Also, it was confirmed that repeated treatment of periodontitis rats with NCP effectively reduced the inflammatory cells and osteoclast activity. As a result, this study suggests that NCP can be directly helpful in the treatment of periodontitis in the future.</jats:p>","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38892350","pmcid":"PMC11172587","openalex_id":"https://openalex.org/W4399284365","authors":[],"funders":[],"total_grants":0,"fwci":2.2036,"citation_percentile":0.87138648,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/1422-0067/25/11/6161/pdf?version=1717423190","host_type":"journal"},{"url":"https://www.mdpi.com/1422-0067/25/11/6161/pdf?version=1717423190","host_type":"publisher"},{"url":"https://www.mdpi.com/1422-0067/25/11/6161/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/ijms25116161","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38892350","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11172587","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/ijms25116161","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11172587/pdf/ijms-25-06161.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11172587","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11172587?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Oral microbiology and periodontitis research","Medical and Biological Ozone Research","Laser Applications in Dentistry and Medicine","Animals","Porphyromonas gingivalis","Periodontitis","Lipopolysaccharides","Rats","Anti-Inflammatory Agents","Humans","Gingiva","Fibroblasts","Ozone","Plasma Gases","Male","Cytokines","Disease Models, Animal","Nitric Oxide","Cells, Cultured"],"mesh_terms":["Animals","Anti-Inflammatory Agents","Cells, Cultured","Disease Models, Animal","Fibroblasts","Gingiva","Humans","Lipopolysaccharides","Male","Nitric Oxide","Ozone","Periodontitis","Cytokines","Porphyromonas gingivalis","Rats","Plasma Gases"],"keywords":["Porphyromonas gingivalis","Periodontitis","Lipopolysaccharide","Dental alveolus","Inflammation","Osteoclast","Chemistry","Anti-inflammatory","Periodontal fiber","Medicine","Immunology","Dentistry","Biochemistry","In vitro","Electron","No-ozone Cold Plasma"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T13:48:59.689087Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}