{"doi":"10.3390/ijms25010380","title":"Comparative Analysis of Differential Cellular Transcriptome and Proteome Regulation by HIV-1 and HIV-2 Pseudovirions in the Early Phase of Infection","abstract":"In spite of the similar structural and genomic organization of human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2), striking differences exist between them in terms of replication dynamics and clinical manifestation of infection. Although the pathomechanism of HIV-1 infection is well characterized, relatively few data are available regarding HIV-2 viral replication and its interaction with host-cell proteins during the early phase of infection. We utilized proteo-transcriptomic analyses to determine differential genome expression and proteomic changes induced by transduction with HIV-1/2 pseudovirions during 8, 12 and 26 h time-points in HEK-293T cells. We show that alteration in the cellular milieu was indeed different between the two pseudovirions. The significantly higher number of genes altered by HIV-2 in the first two time-points suggests a more diverse yet subtle effect on the host cell, preparing the infected cell for integration and latency. On the other hand, GO analysis showed that, while HIV-1 induced cellular oxidative stress and had a greater effect on cellular metabolism, HIV-2 mostly affected genes involved in cell adhesion, extracellular matrix organization or cellular differentiation. Proteomics analysis revealed that HIV-2 significantly downregulated the expression of proteins involved in mRNA processing and translation. Meanwhile, HIV-1 influenced the cellular level of translation initiation factors and chaperones. Our study provides insight into the understudied replication cycle of HIV-2 and enriches our knowledge about the use of HIV-based lentiviral vectors in general.","journal":"International Journal of Molecular Sciences","year":2023,"id":378256,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9412,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":674752,"name":"Viktor Ambrus","orcid":null,"position":1,"is_corresponding":false},{"id":674163,"name":"Balázs Kunkli","orcid":"0000-0003-1266-2792","position":2,"is_corresponding":false},{"id":674164,"name":"Zsófia Ilona Szojka","orcid":"0000-0002-1252-6929","position":3,"is_corresponding":false},{"id":674165,"name":"Gergő Kalló","orcid":"0000-0002-5732-8758","position":4,"is_corresponding":false},{"id":674166,"name":"Éva Csősz","orcid":"0000-0003-4373-2175","position":5,"is_corresponding":false},{"id":674167,"name":"Ajneesh Kumar","orcid":"0000-0001-5513-4135","position":6,"is_corresponding":false},{"id":674168,"name":"Miklós Emri","orcid":"0000-0001-9884-9202","position":7,"is_corresponding":false},{"id":337322,"name":"József Tőzsér","orcid":"0000-0001-5076-8729","position":8,"is_corresponding":false},{"id":337321,"name":"Mohamed Mahdi","orcid":"0000-0002-7959-5996","position":9,"is_corresponding":false},{"id":674751,"name":"Tamás Richárd Linkner","orcid":null,"position":0,"is_corresponding":true}],"reference_count":110,"raw_metadata":null,"created_at":"2026-07-19T01:16:44.708019Z","pmid":"38203551","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}