{"doi":"10.3390/ijms242417169","title":"Knockouts of TNFRSF1A and TNFRSF1B Genes in K562 Cell Line Lead to Diverse Long-Lasting Responses to TNF-α","abstract":"<jats:p>This research delves into the intricate landscape of tumor necrosis factor-alpha (TNF-α) signaling, a multi-functional cytokine known for its diverse cellular effects. Specifically, we investigate the roles of two TNF receptors, TNFR1 and TNFR2, in mediating TNF-α-induced transcriptional responses. Using human K562 cell lines with TNFR1 and TNFR2 knockouts, we explore changes in gene expression patterns following TNF-α stimulation. Our findings reveal distinct transcriptional profiles in TNFR1 and TNFR2 knockout cells, shedding light on the unique contributions of these receptors to TNF-α signaling. Notably, several key pathways associated with inflammation, apoptosis, and cell proliferation exhibit altered regulation in the absence of TNFR1 or TNFR2. This study provides valuable insights into the intricate mechanisms governing TNF-α signaling and its diverse cellular effects, with potential implications for targeted therapeutic strategies.</jats:p>","journal":"International Journal of Molecular Sciences","year":2023,"id":689797,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1802064,"name":"Saleh Alrhmoun","orcid":"0000-0003-0788-5385","position":1,"is_corresponding":false},{"id":1802065,"name":"Roman Perik-Zavodskii","orcid":"0000-0002-5055-4859","position":2,"is_corresponding":false},{"id":1802066,"name":"Julia Zhukova","orcid":null,"position":3,"is_corresponding":false},{"id":1802067,"name":"Julia Lopatnikova","orcid":null,"position":4,"is_corresponding":false},{"id":1802068,"name":"Marina Volynets","orcid":"0000-0003-1683-8951","position":5,"is_corresponding":false},{"id":1802069,"name":"Alina Alshevskaya","orcid":null,"position":6,"is_corresponding":false},{"id":1570538,"name":"Sergey Sennikov","orcid":"0000-0002-7366-7768","position":7,"is_corresponding":false},{"id":1802063,"name":"Olga Perik-Zavodskaia","orcid":"0000-0003-4911-1994","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Knockouts of TNFRSF1A and TNFRSF1B Genes in K562 Cell Line Lead to Diverse Long-Lasting Responses to TNF-α","abstract":"<jats:p>This research delves into the intricate landscape of tumor necrosis factor-alpha (TNF-α) signaling, a multi-functional cytokine known for its diverse cellular effects. Specifically, we investigate the roles of two TNF receptors, TNFR1 and TNFR2, in mediating TNF-α-induced transcriptional responses. Using human K562 cell lines with TNFR1 and TNFR2 knockouts, we explore changes in gene expression patterns following TNF-α stimulation. Our findings reveal distinct transcriptional profiles in TNFR1 and TNFR2 knockout cells, shedding light on the unique contributions of these receptors to TNF-α signaling. Notably, several key pathways associated with inflammation, apoptosis, and cell proliferation exhibit altered regulation in the absence of TNFR1 or TNFR2. This study provides valuable insights into the intricate mechanisms governing TNF-α signaling and its diverse cellular effects, with potential implications for targeted therapeutic strategies.</jats:p>","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38138998","pmcid":"PMC10742831","openalex_id":"https://openalex.org/W4389397834","authors":[],"funders":[{"funder_name":"Russian Science Foundation","grant_id":"20-75-10051","title":null},{"funder_name":"State task","grant_id":"122011800353-4","title":null}],"total_grants":2,"fwci":0.7178,"citation_percentile":0.68592572,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2025,"count":5},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/1422-0067/24/24/17169/pdf?version=1701833957","host_type":"journal"},{"url":"https://www.mdpi.com/1422-0067/24/24/17169/pdf?version=1701833957","host_type":"GOLD"},{"url":"https://www.mdpi.com/1422-0067/24/24/17169/pdf?version=1701833957","host_type":"publisher"},{"url":"https://www.mdpi.com/1422-0067/24/24/17169/pdf","host_type":"publisher"},{"url":"https://doi.org/10.3390/ijms242417169","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38138998","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10742831","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10742831/pdf/ijms-24-17169.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10742831","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10742831?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Immune Response and Inflammation","NF-κB Signaling Pathways","interferon and immune responses","Medicine","Biology","Humans","Receptors, Tumor Necrosis Factor, Type I","Tumor Necrosis Factor-alpha","Receptors, Tumor Necrosis Factor, Type II","K562 Cells","Cytokines"],"mesh_terms":["Humans","Tumor Necrosis Factor-alpha","Cytokines","K562 Cells","Receptors, Tumor Necrosis Factor, Type I","Receptors, Tumor Necrosis Factor, Type II"],"keywords":["Tumor necrosis factor alpha","Gene knockout","Cell biology","Signal transduction","Biology","K562 cells","Cytokine","Receptor","Tumor necrosis factor receptor 1","Apoptosis","Knockout mouse","Cell culture","Gene","Immunology","Tumor necrosis factor receptor","Genetics","Knockout","K562","TNFR1","Gene Expression","Cell Line Model","Tnfrsf1a","Tnfr2","Tnfrsf1b"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-22T16:00:35.238857Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}