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We show that ischemia induces specific changes in the short-chain acyl-CoA relative concentrations, while mild ischemia (1–2 min), although reducing succinyl-CoA, has little effects on acetyl-CoA, and even increases some acyl-CoA species upstream of the tricarboxylic acid cycle. In contrast, advanced ischemia (5–6 min) also reduces acetyl-CoA levels. Our approach provides the keys to accessing the acyl-CoA metabolome for a more in-depth analysis of metabolism, protein acylation and epigenetics.</jats:p>","journal":"International Journal of Molecular Sciences","year":2023,"id":685423,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1790711,"name":"Nour Zeaiter","orcid":null,"position":1,"is_corresponding":false},{"id":1790712,"name":"Valérie Cunin","orcid":null,"position":2,"is_corresponding":false},{"id":1790713,"name":"Stéphane Attia","orcid":null,"position":3,"is_corresponding":false},{"id":1790714,"name":"Cécile Meunier","orcid":null,"position":4,"is_corresponding":false},{"id":1790715,"name":"Laurence Kay","orcid":null,"position":5,"is_corresponding":false},{"id":1790716,"name":"Amel Achouri","orcid":null,"position":6,"is_corresponding":false},{"id":1790717,"name":"Edwige Hiriart-Bryant","orcid":null,"position":7,"is_corresponding":false},{"id":1790718,"name":"Karine Couturier","orcid":null,"position":8,"is_corresponding":false},{"id":1790719,"name":"Cindy Tellier","orcid":null,"position":9,"is_corresponding":false},{"id":1790720,"name":"Abderrafek El Harras","orcid":null,"position":10,"is_corresponding":false},{"id":1790721,"name":"Bénédicte Elena-Herrmann","orcid":"0000-0002-0230-1590","position":11,"is_corresponding":false},{"id":659563,"name":"Saadi Khochbin","orcid":"0000-0002-0455-0857","position":12,"is_corresponding":false},{"id":105869,"name":"Audrey Le Gouëllec","orcid":"0000-0002-4905-3854","position":13,"is_corresponding":false},{"id":830118,"name":"Uwe Schlattner","orcid":"0000-0003-1159-5911","position":14,"is_corresponding":false},{"id":1790710,"name":"Malgorzata Tokarska-Schlattner","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Multi-Method Quantification of Acetyl-Coenzyme A and Further Acyl-Coenzyme A Species in Normal and Ischemic Rat Liver","abstract":"<jats:p>Thioesters of coenzyme A (CoA) carrying different acyl chains (acyl-CoAs) are central intermediates of many metabolic pathways and donor molecules for protein lysine acylation. Acyl-CoA species largely differ in terms of cellular concentrations and physico-chemical properties, rendering their analysis challenging. Here, we compare several approaches to quantify cellular acyl-CoA concentrations in normal and ischemic rat liver, using HPLC and LC-MS/MS for multi-acyl-CoA analysis, as well as NMR, fluorimetric and spectrophotometric techniques for the quantification of acetyl-CoAs. In particular, we describe a simple LC-MS/MS protocol that is suitable for the relative quantification of short and medium-chain acyl-CoA species. We show that ischemia induces specific changes in the short-chain acyl-CoA relative concentrations, while mild ischemia (1–2 min), although reducing succinyl-CoA, has little effects on acetyl-CoA, and even increases some acyl-CoA species upstream of the tricarboxylic acid cycle. In contrast, advanced ischemia (5–6 min) also reduces acetyl-CoA levels. 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