{"doi":"10.3390/ijms241512375","title":"Inhibition of CDK1 by RO-3306 Exhibits Anti-Tumorigenic Effects in Ovarian Cancer Cells and a Transgenic Mouse Model of Ovarian Cancer","abstract":"Ovarian cancer is the deadliest gynecological malignancy of the reproductive organs in the United States. Cyclin-dependent kinase 1 (CDK1) is an important cell cycle regulatory protein that specifically controls the G2/M phase transition of the cell cycle. RO-3306 is a selective, ATP-competitive, and cell-permeable CDK1 inhibitor that shows potent anti-tumor activity in multiple pre-clinical models. In this study, we investigated the effect of CDK1 expression on the prognosis of patients with ovarian cancer and the anti-tumorigenic effect of RO-3306 in both ovarian cancer cell lines and a genetically engineered mouse model of high-grade serous ovarian cancer (KpB model). In 147 patients with epithelial ovarian cancer, the overexpression of CDK1 was significantly associated with poor prognosis compared with a low expression group. RO-3306 significantly inhibited cellular proliferation, induced apoptosis, caused cellular stress, and reduced cell migration. The treatment of KpB mice with RO-3306 for four weeks showed a significant decrease in tumor weight under obese and lean conditions without obvious side effects. Overall, our results demonstrate that the inhibition of CDK1 activity by RO-3306 effectively reduces cell proliferation and tumor growth, providing biological evidence for future clinical trials of CDK1 inhibitors in ovarian cancer.","journal":"International Journal of Molecular Sciences","year":2023,"id":337505,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9531,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":498612,"name":"Yali Fan","orcid":null,"position":1,"is_corresponding":false},{"id":1038371,"name":"Ziyi Zhao","orcid":"0000-0003-1871-5197","position":2,"is_corresponding":false},{"id":340227,"name":"Xin Zhang","orcid":"0000-0001-6036-2685","position":3,"is_corresponding":false},{"id":341750,"name":"Katherine L. Tucker","orcid":"0000-0001-7640-662X","position":4,"is_corresponding":false},{"id":498613,"name":"Allison Staley","orcid":null,"position":5,"is_corresponding":false},{"id":1038780,"name":"Hongyan Suo","orcid":null,"position":6,"is_corresponding":false},{"id":466465,"name":"Wenchuan Sun","orcid":null,"position":7,"is_corresponding":false},{"id":1038781,"name":"Xiaochang Shen","orcid":null,"position":8,"is_corresponding":false},{"id":1038369,"name":"Boer Deng","orcid":"0000-0002-4833-9244","position":9,"is_corresponding":false},{"id":589927,"name":"Stuart R. Pierce","orcid":null,"position":10,"is_corresponding":false},{"id":589928,"name":"Lindsay West","orcid":null,"position":11,"is_corresponding":false},{"id":21651,"name":"Yajie Yin","orcid":"0000-0002-2220-005X","position":12,"is_corresponding":false},{"id":268429,"name":"Michael J. Emanuele","orcid":"0000-0003-4104-7449","position":13,"is_corresponding":false},{"id":465345,"name":"Chunxiao Zhou","orcid":"0000-0002-1257-9537","position":14,"is_corresponding":false},{"id":465346,"name":"Victoria Bae‐Jump","orcid":"0000-0001-6778-0951","position":15,"is_corresponding":false},{"id":497997,"name":"Yu Huang","orcid":"0000-0001-7285-3692","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-19T01:10:26.690308Z","pmid":"37569750","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}