{"doi":"10.3390/ijms24097826","title":"NF-κB Signaling Modulates miR-452-5p and miR-335-5p Expression to Functionally Decrease Epithelial Ovarian Cancer Progression in Tumor-Initiating Cells","abstract":"Epithelial ovarian cancer (EOC) remains the fifth leading cause of cancer-related death in women worldwide, partly due to the survival of chemoresistant, stem-like tumor-initiating cells (TICs) that promote disease relapse. We previously described a role for the NF-κB pathway in promoting TIC chemoresistance and survival through NF-κB transcription factors (TFs) RelA and RelB, which regulate genes important for the inflammatory response and those associated with cancer, including microRNAs (miRNAs). We hypothesized that NF-κB signaling differentially regulates miRNA expression through RelA and RelB to support TIC persistence. Inducible shRNA was stably expressed in OV90 cells to knockdown RELA or RELB; miR-seq analyses identified differentially expressed miRNAs hsa-miR-452-5p and hsa-miR-335-5p in cells grown in TIC versus adherent conditions. We validated the miR-seq findings via qPCR in TIC or adherent conditions with RELA or RELB knocked-down. We confirmed decreased expression of hsa-miR-452-5p when either RELA or RELB were depleted and increased expression of hsa-miR-335-5p when RELA was depleted. Either inhibiting miR-452-5p or mimicking miR-335-5p functionally decreased the stem-like potential of the TICs. These results highlight a novel role of NF-κB TFs in modulating miRNA expression in EOC cells, thus opening a better understanding toward preventing recurrence of EOC.","journal":"International Journal of Molecular Sciences","year":2023,"id":362088,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9625,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":464151,"name":"Brittney S. Harrington","orcid":"0000-0001-7083-069X","position":1,"is_corresponding":false},{"id":1115739,"name":"Emma Attar","orcid":null,"position":2,"is_corresponding":false},{"id":670248,"name":"Soumya Korrapati","orcid":null,"position":3,"is_corresponding":false},{"id":369484,"name":"Jyoti Shetty","orcid":"0000-0003-1496-4057","position":4,"is_corresponding":false},{"id":228426,"name":"Yongmei Zhao","orcid":"0000-0003-0800-4658","position":5,"is_corresponding":false},{"id":271310,"name":"Bao Tran","orcid":"0000-0002-0261-4458","position":6,"is_corresponding":false},{"id":342513,"name":"Nathan Wong","orcid":"0000-0002-8882-3401","position":7,"is_corresponding":false},{"id":464154,"name":"Carrie D. House","orcid":"0000-0002-1870-5872","position":8,"is_corresponding":false},{"id":230687,"name":"Christina M. Annunziata","orcid":"0000-0003-2033-6532","position":9,"is_corresponding":false},{"id":892559,"name":"Rahul Kamdar","orcid":null,"position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:14:19.266946Z","pmid":"37175530","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}