{"doi":"10.3390/ijms24076433","title":"Mouse Spinal Cord Vascular Transcriptome Analysis Identifies CD9 and MYLIP as Injury-Induced Players","abstract":"<jats:p>Traumatic spinal cord injury (SCI) initiates a cascade of cellular events, culminating in irreversible tissue loss and neuroinflammation. After the trauma, the blood vessels are destroyed. The blood-spinal cord barrier (BSCB), a physical barrier between the blood and spinal cord parenchyma, is disrupted, facilitating the infiltration of immune cells, and contributing to a toxic spinal microenvironment, affecting axonal regeneration. Understanding how the vascular constituents of the BSCB respond to injury is crucial to prevent BSCB impairment and to improve spinal cord repair. Here, we focus our attention on the vascular transcriptome at 3- and 7-days post-injury (dpi), during which BSCB is abnormally leaky, to identify potential molecular players that are injury-specific. Using the mouse contusion model, we identified Cd9 and Mylip genes as differentially expressed at 3 and 7 dpi. CD9 and MYLIP expression were injury-induced on vascular cells, endothelial cells and pericytes, at the injury epicentre at 7 dpi, with a spatial expression predominantly at the caudal region of the lesion. These results establish CD9 and MYLIP as two new potential players after SCI, and future studies targeting their expression might bring promising results for spinal cord repair.</jats:p>","journal":"International Journal of Molecular Sciences","year":2023,"id":632509,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1639596,"name":"Dalila Neves-Silva","orcid":"0000-0003-2342-5354","position":1,"is_corresponding":false},{"id":521119,"name":"Mariana Ascensão-Ferreira","orcid":"0000-0002-5247-246X","position":2,"is_corresponding":false},{"id":1639600,"name":"Ana Filipa Dias","orcid":null,"position":3,"is_corresponding":false},{"id":1558770,"name":"Daniel Ribeiro","orcid":"0000-0003-3660-9646","position":4,"is_corresponding":false},{"id":1639601,"name":"Ana Filipa Isidro","orcid":null,"position":5,"is_corresponding":false},{"id":1639603,"name":"Raquel Quitéria","orcid":null,"position":6,"is_corresponding":false},{"id":1639605,"name":"Diogo Paramos-de-Carvalho","orcid":null,"position":7,"is_corresponding":false},{"id":169500,"name":"Nuno L. Barbosa-Morais","orcid":null,"position":8,"is_corresponding":false},{"id":885991,"name":"Leonor Saúde","orcid":"0000-0001-5933-8872","position":9,"is_corresponding":false},{"id":1639594,"name":"Isaura Martins","orcid":"0000-0002-1329-7769","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mouse Spinal Cord Vascular Transcriptome Analysis Identifies CD9 and MYLIP as Injury-Induced Players","abstract":"<jats:p>Traumatic spinal cord injury (SCI) initiates a cascade of cellular events, culminating in irreversible tissue loss and neuroinflammation. After the trauma, the blood vessels are destroyed. The blood-spinal cord barrier (BSCB), a physical barrier between the blood and spinal cord parenchyma, is disrupted, facilitating the infiltration of immune cells, and contributing to a toxic spinal microenvironment, affecting axonal regeneration. Understanding how the vascular constituents of the BSCB respond to injury is crucial to prevent BSCB impairment and to improve spinal cord repair. Here, we focus our attention on the vascular transcriptome at 3- and 7-days post-injury (dpi), during which BSCB is abnormally leaky, to identify potential molecular players that are injury-specific. Using the mouse contusion model, we identified Cd9 and Mylip genes as differentially expressed at 3 and 7 dpi. CD9 and MYLIP expression were injury-induced on vascular cells, endothelial cells and pericytes, at the injury epicentre at 7 dpi, with a spatial expression predominantly at the caudal region of the lesion. These results establish CD9 and MYLIP as two new potential players after SCI, and future studies targeting their expression might bring promising results for spinal cord repair.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37047406","pmcid":"PMC10094762","openalex_id":null,"authors":[],"funders":[{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"PTDC/BIM-MED/3295/2014","title":"Angiocrine factors as modulators of spinal cord regeneration"},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"IF/00595/2014","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"LISBOA-01-0145-FEDER-007391","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"HR18-00187","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"2021.02253.CEECIND","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"EMBO Installation Grant 3057","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"CEECIND/00436/2018","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"PTDC/EMS-SIS/0642/2014","title":"PERSEIDS - Personalizing cancer therapy through integrated modeling and decision"},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"SFRH/BPD/118051/2016","title":"Angiocrine factors as modulators of spinal cord regeneration"},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"SFRH/BD/138636/2018","title":"Using the regenerative potential of zebrafish extracellular matrix to promote spinal cord repair in mammals."},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"PD/BD/128283/2017","title":"When introns behave like exons: intron retention as a mechanism for dynamic regulation of gene expression"},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"COVID/BD/151620/2021","title":"When introns behave like exons: intron retention as a mechanism for dynamic regulation of gene expression"},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"2020.08168.BD","title":"The role of induced-senescent cells in spinal cord injury"},{"funder_name":"Fundação para a Ciência e a Tecnologia","grant_id":"PD/BD/105770/2014","title":"A DEFINIR"}],"total_grants":14,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.mdpi.com/1422-0067/24/7/6433/pdf?version=1680091253","host_type":"publisher"},{"url":"https://www.mdpi.com/1422-0067/24/7/6433/pdf","host_type":"publisher"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10094762/pdf/ijms-24-06433.pdf","host_type":"repository"},{"url":"https://dx.doi.org/10.3390/ijms24076433","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10094762","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10094762?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.3390/ijms24076433","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/37047406","host_type":""},{"url":"http://dx.doi.org/10.3390/ijms24076433","host_type":""}],"fields_of_study":[],"mesh_terms":["Blood-Brain Barrier","Pericytes","Spinal Cord","Endothelial Cells","Animals","Mice","Spinal Cord Injuries","Disease Models, Animal","Gene Expression Profiling"],"keywords":["Spinal cord injury","Transcriptome","Endothelial Cells","Pericytes","Perivascular Cells","Blood Spinal Cord Barrier","Gene Expression Profiling","Article","Mice","Disease Models, Animal","Spinal Cord","Blood-Brain Barrier","Animals","Spinal Cord Injuries"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T09:53:34.069364Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}