{"doi":"10.3390/ijms232012416","title":"mTOR Contributes to the Proteome Diversity through Transcriptome-Wide Alternative Splicing","abstract":"The mammalian target of rapamycin (mTOR) pathway is crucial in energy metabolism and cell proliferation. Previously, we reported transcriptome-wide 3'-untranslated region (UTR) shortening by alternative polyadenylation upon mTOR activation and its impact on the proteome. Here, we further interrogated the mTOR-activated transcriptome and found that hyperactivation of mTOR promotes transcriptome-wide exon skipping/exclusion, producing short isoform transcripts from genes. This widespread exon skipping confers multifarious regulations in the mTOR-controlled functional proteomics: AS in coding regions widely affects the protein length and functional domains. They also alter the half-life of proteins and affect the regulatory post-translational modifications. Among the RNA processing factors differentially regulated by mTOR signaling, we found that SRSF3 mechanistically facilitates exon skipping in the mTOR-activated transcriptome. This study reveals a role of mTOR in AS regulation and demonstrates that widespread AS is a multifaceted modulator of the mTOR-regulated functional proteome.","journal":"International Journal of Molecular Sciences","year":2022,"id":262501,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":569817,"name":"Naima Ahmed Fahmi","orcid":"0000-0003-2745-2130","position":1,"is_corresponding":false},{"id":301005,"name":"Mee Yeon Park","orcid":"0000-0001-8247-3925","position":2,"is_corresponding":false},{"id":380220,"name":"Jiao Sun","orcid":"0000-0002-7480-025X","position":3,"is_corresponding":false},{"id":570459,"name":"Kaitlyn Thao","orcid":null,"position":4,"is_corresponding":false},{"id":418535,"name":"Hsin-Sung Yeh","orcid":"0000-0002-0695-8055","position":5,"is_corresponding":false},{"id":380219,"name":"Wei Zhang","orcid":"0000-0003-3605-9373","position":6,"is_corresponding":false},{"id":380221,"name":"Jeongsik Yong","orcid":"0000-0002-2758-0450","position":7,"is_corresponding":false},{"id":569816,"name":"Sze Cheng","orcid":"0000-0002-0102-9812","position":0,"is_corresponding":true}],"reference_count":76,"raw_metadata":null,"created_at":"2026-07-19T00:26:25.074177Z","pmid":"36293270","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}